摘要
该研究探讨了大蒜素诱导人胃癌SGC-7901细胞凋亡及其作用机制。用不同浓度大蒜素作用人胃癌SGC-7901细胞48 h。通过MTT法检测细胞活性。光学、激光共聚焦显微镜下观察细胞形态变化。流式细胞术检测细胞凋亡率和细胞周期。qRT-PCR和Western blot检测Bax、Bcl-2基因和蛋白的表达水平。结果显示,大蒜素处理细胞48 h的IC50值为78μg/m L,显微镜下可观察到明显的凋亡现象,细胞凋亡率为(61.15±3.77)%,细胞阻滞于G1期,Bcl-2基因和蛋白表达均下降,Bax基因和蛋白表达均增加(P<0.05)。综上所述,在一定浓度范围内,大蒜素能抑制人胃癌SGC-7901细胞增殖,诱导细胞凋亡,呈剂量依赖性,并可上调Bax基因表达,下调Bcl-2基因表达。
This work was aimed to investigate the apoptosis-inducing effect of allicin in human gastric cancer SGC-7901 cells and analyze its molecular mechanisms. MTT assay was used to determine SGC-7901 cells activity. Morphological changes of cells were observed under optical and laser confocal microscope. Apoptosis rate and cell cycle distribution were detected by flow cytometry. The expressions of Bax and Bcl-2 were measured by q RT-PCR and Western blot. The results showed that the IC50 was 78 μg/m L after the SGC-7901 cells treated with allicin for 48 h in this study. The cells treated with allicin showed a typical apoptotic morphology. The apoptosis rate was up to（61.15±3.77）% and the G1 arrest was induced significantly after treated with allicin. The results suggested that allicin inhibited the proliferation of SGC-7901 cells in a dose-dependent manner, and induced the apoptosis of gastric cancer cells through up-regulation of Bax and down-regulation of Bc1-2.
作者
朱少伟
邵淑丽
张蕾
张伟伟
陈琳
刘畅
Zhu Shaowei Shao Shuli Zhang Lei Zhang Weiwei Chen Lin Liu Chang(College of Life Sciences and Agriculture and Forestry, Qiqihar University, Qiqihar 161000, China)
出处
《中国细胞生物学学报》
CAS
CSCD
2017年第1期6-12,共7页
Chinese Journal of Cell Biology
基金
黑龙江省自然科学基金(批准号:C201241)
黑龙江省教育厅科学技术项目(批准号:12511611)
齐齐哈尔大学研究生创新项目(批准号:YJSCX2016-ZD09)资助的课题~~
