摘要
目的:探讨大蒜素诱导人胃癌SGC-7901细胞M期阻滞作用及其机制。方法:以MTT比色法检测人胃癌细胞系SGC-7901细胞的增殖抑制率;采用免疫荧光染色、激光共聚焦显微镜检测等技术,比较药物处理前后M期细胞占总细胞数百分比的变化,观察细胞微管结构及细胞周期蛋白B1(cyclin B1)在胞内分布的变化,并做荧光定量分析cyclin B1在用药前后量的改变。结果:大蒜素抑制SGC-7901细胞的24h IC50为7.2μg/ml,72h IC50为20μg/ml;经大蒜素处理后,M期细胞占总细胞数的30.61%,明显高于对照组的4.69%(P<0.05)。经大蒜素处理后细胞变小变圆,微管结构消失,胞质模糊;而对照组细胞形态正常,微管结构清晰完整。经大蒜素处理后,胞质中cyclin B1表达升高,并向核内聚集,cyclin B1荧光定量明显高于对照组(P<0.01)。结论:大蒜素可以促进微管解聚,可以提高促成熟因子(MPF)的重要成份cyclin B1在细胞内的表达并促进其向核内转移,使细胞周期阻滞于M期。大蒜素可以作为一种新的作用于M期的抗肿瘤药物应用于临床。
Objective：To determine the effect of allitridi on inducing mitotic arrest in human gastric cell line SGC-7901,and its possible mechanisms.Methods：The SGC-7901 cells were treated with allitridi.Proliferation inhibitory rate was detected by MTT colormetric assay.We used immunofluorescence and confocal laser scanning microscope technique to observe the morphologic changes of the microtubule structure and the location of cyclin B1.Leica confocal software was used to quantitately analyze the express of cyclin B1.Results：The SGC-7901 cells were inhibited after exposure to allitridi for 24 h,the IC50 was 7.2 μg/ml,for 72 h,the IC50 was 20 μg/ml.After being treated with allitridi the percentage of mitotic cells is significantly exceed to the control.The treated cells became more shrunken and nepheloid,the microtubule networks in them disappeared and the cyclin B1 protein was expressed more significantly and was concentrated in the nucleus.The fluorescence intensity of the cyclin B1 protein in treated cells was much more significant than control cell’s.Conclusion：The allitridi can render arrest of SGC-7901 cells in M phase,and one of its possible mechanisms is due to enhancing microtubule depolymerization by elevating the expression of cyclin B1.Allitridi might be a new M phase specific anticancer drugs.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2007年第6期686-688,共3页
Journal of China Medical University
基金
国家自然科学基金资助项目(30572131)
