摘要
目的观察姜黄素对急性肺栓塞（APE）大鼠B型钠尿肽（BNP）、心肌肌钙蛋白I（cTnI）、D-二聚体的影响。方法设计合成不规则趋化因子（CX3CL1）-短发夹RNA（shRNA）和过表达载体，分别进行腺病毒包装。将60只SD大鼠按随机数字表法分为正常对照组、假手术组、模型组、姜黄素组、姜黄素＋shRNA组、姜黄素＋CX3CL1过表达载体组，每组10只。用自体血栓复制方法制备APE动物模型，药物干预组于制模前1d和40min分别灌胃姜黄素100mg／kg1次；病毒干预组于制模前3d尾静脉注射10^9pfu1次；假手术组和模型组灌胃等量生理盐水2mL，每天1次；正常对照组不接受任何干预。制模后6h，取肺组织，激光共聚焦显微镜下观察肺组织变化，苏木素-伊红（HE）染色，光镜下观察肺组织病理学改变；用酶联免疫吸附试验（ELISA）检测血清BNP、cTnI、D-二聚体水平。结果病毒感染肺组织呈绿色，细胞核呈蓝色。肺组织病理学观察显示：模型组较正常对照组及假手术组肺组织病理学改变明显，经姜黄素干预后，各治疗组肺动脉内部分栓塞溶解，炎症减轻。ELISA检测结果显示：与假手术组比较，模型组大鼠血清cTnI、BNP、D-二聚体含量显著升高[cTnI（ng／L）：224．97±37．96比149．13±36．65，BNP（ng／L）：53．66±21．72比24．62±10．44，D-二聚体（ng／L）：78．68±13．10比42．00±930，均P〈0．05]；与模型组比较，姜黄素组、姜黄素＋shRNA组和姜黄素＋CX3CL1过表达载体组血清cTnI、BNP、D-二聚体含量显著降低（cTnI为162．98±28．65、143．32±34．55、159．50±37．80比224．97±37．96，均P〈0．05，BNP为27．45±11．00、26．95±6．66、33．05±7．05比53．66±21．72，均P〈0．05，D-二聚体为54．34±4．57、53．38±11．01、56．33±6．80比78．68±13．10，均P〈0．05）；各干预组间比较差异均无统计学意义（均P〉0．05）。�
Objective To observe the effects of curcumin on B-type natriuretic peptide （BNP）, cardiac troponin I （cTnI） and D-dimer in rats with acute pulmonary embolism （APE）. Methods The irregular ehemotaetic factor （CX3CL1）-shRNA and overexpression vector were designed and synthesized, and adenovirus packages were respectively carried out. Sixty Spargue-Dawley （SD） rats were divided into normal control group, sham-operation group, model group, curcumin group, curcumin＋CX3CL1shRNA group and curcumin＋CX3CL1overexpression vector group by random number table （each group 10 rats）. The APE rat models were duplicated by autologous blood embolus method, in the pharmacological intervention groups, 1 day and again 40 minutes before operation, a dose of curcumin 100 mg/kg was administered by gavage respectively; in virus intervention groups, 3 days before modeling, intravenous injection of 109 pfu into a tail vein was performed once; in sham-operation group and model group, an equivalent dose of normal saline 2 mL daily was administered by garage; the normal group did not accept any intervention. Six hours after modeling, the change of lung tissue were observed under confocal laser scanning microscope, the lung tissue was taken for hematoxylin-eosin （HE） staining, histopathological changes of lung tissue were observed, and the levels of serum BNP, cTnI, D-dimer were detected by enzyme-linked immunosorbent assay （ELISA）. Results Virus infected lung tissue was green, nucler was blue. The HE staining in lung tissue showed： histopathological changes in the model group were more obvious than those in normal group and sham-operation group, after curcumin intervention, some emboli in pulmonary arteries in all treatment groups were partially dissolved, and the inflammation was alleviated. The results of ELISA shows： the contents of cTnI, BNP, D-dimer were significantly higher in model group than those in shamoperation group [cTnI （ng/L）： 224.97 ± 37.96 vs. 149.13 ± 36.65, BNP （ng/L）： 53.
作者
朱春海
邝晶
王灵聪
Zhu Chunhai Kuang Jing Wang Lingcong(Qingtian Traditional Chinese Medicine Hospital, Lishui 323900, Zhejiang, China the First Affiliated Hospital of Zhejiang Traditional Chinese Medical University, Hangzhou 310006, Zhejiang, China)
出处
《中国中西医结合急救杂志》
CAS
CSCD
北大核心
2017年第1期78-81,共4页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
浙江省自然科学基金（LY17H290006,LY12H29005）
浙江省医药卫生平台计划重点资助项目（2015ZDA022）
浙江省卫生高层次创新人才培养工程资助项目（2014-108）
