摘要
为了获得性状优良的水稻种质资源,采用EMS方法对缙恢21进行诱变,M4代获得不同类型的稳定突变材料,选取36份性状明显不同的突变材料进行SSR分子标记分析。从覆盖水稻12对染色体的200对SSR引物中筛选出15对扩增产物具有稳定多态性的引物,检测到59个等位基因,有效等位基因数为46．2574,有效等位基因百分数为78．40%,每对引物检测出3～5个等位基因,平均为3．9333个；遗传多样性指数在0．9951～1．3849之间,平均为1．2071；每个位点的多态性信息量变化于0．8285～0．9794之间,平均为0．9134。聚类分析得出,36份材料的遗传相似系数变幅为0．515～1．000,并在遗传相似系数0．63处将36份材料分为两大类群。
In order to obtain good traits of rice germplasm resources, the EMS method was used to mutagenize rice Jin-hui21. Different types of stable mutants were obtained in M4 generation. Thirty-six mutants with obvious different traits were selected for SSR molecular marker analysis. Fifteen pairs of primers with stable polymorphism were screened out from 200 pairs of SSR primers covering 12 chromosomes of rice. Fifty-nine alleles were detected, effective number of alleles was 46.2574, and effective allele percentage was 78.40%. The average number of alleles per SSR locus was 3.9333 with a range of three to five. The genetic diversity index varied from 0.9951 to 1.3849 with an average of 0.9134. The polymor-phism information content for the SSR loci varied from 0.8285 to 0.9794 with an average of 0.9134. Genetic similarities a-mong the 36 mutant materials ranged from 0.515 to 1.000. The 36 mutant materials could be divided into 2 groups by u-sing the genetic similarity coefficient of 0.63.
作者
张修宝
罗洪发
查仁明
冯仕飞
樊海容
张姗姗
庹中丽
ZHANG Xiu-bao LUO Hong-fa ZHA Ren-ming FENG Shi-fei ZHANG Shan-shan DUO Zhong-li(Guiyang Agricultural Technology Extension Station, Guiyang, Guizhou 550081, China College of Agriculture, Guizhou University, Guiyang, Guizhou 550025, China)
出处
《山地农业生物学报》
2016年第5期30-36,共7页
Journal of Mountain Agriculture and Biology
基金
贵州省科技基金项目"一个新水稻花器官突变体的分子定位研究"(黔科合2007[2052])
贵州省农业攻关项目"红香米有机高产栽培技术及产业化示范研究"(黔科合J字[2010]3005号)
贵州大学校人才引进基金"利用EMS创造水稻新种质及其遗传多样性分析"
关键词
水稻
诱变
遗传差异
SSR
Rice
Mutagenesis
Genetic differences
SSR
