摘要
为了高效分离油茶蒲中的脂肪酸合酶(FAS)抑制剂,以乙醇-水为洗脱液,采用大孔树脂D101对油茶蒲提取物进行梯度洗脱,并用高速逆流色谱(HSCCC)对其中的40%乙醇洗脱馏分(40F)进行分离。结果表明,D101大孔树脂洗脱馏分中40F的多酚含量最高,并且其对脂肪酸合酶的抑制活性最强,半抑制浓度为0.61μg·m L-1。以氯仿∶乙醇∶水∶乙酸(4∶3∶2∶0.01,v/v/v/v)为分离溶剂体系,上相为固定相,下相为流动相,转速850 r·min-1,流速2 m L·min-1,在该条件下采用HSCCC对40F进行分离可以纯化得到鞣花酸和3-O-甲基鞣花酸-4'-O-葡萄糖苷,纯度均为95%以上。因此,采用大孔树脂-高速逆流色谱能够简便、高效地分离制备油茶蒲中的脂肪酸合酶抑制剂单体,这为油茶蒲的开发利用及进一步的功能研究奠定了基础。
In order to separate fatty acid synthase inhibitors in oiltea camellia extracts （OCE） efficiently, combined application of macroporous resin and high-speed counter-current chromatography （HSCCC） was successfully used. Maeroporous resin D101 gradient elution method was used to separate OCE with ethanol-water as eluant, and then 40% ethanol eluted fraction （40F） was separated by HSCCC. The results indicated that the 40F had the highest polyphenol content and the strongest inhibitory effect on fatty acid synthase with an ICs0 of 0.61 r·min-1. Two-phase solvent system of chloroform： ethanol： water： acetic acid （4： 3： 2： 0.01, v/v/v/v） was used in HSCCC for separating 40F at the conditions as follow： upper phase as the stationary phase, lower phase as the mobile phase, revolution 850 r·min-1, flow rate 2.0 r·min-1And ellagic acid and 3 - O-methylellagic acid - 4＇ - O - 13 - D-glucopyranoside were isolated under the conditions with both purity above 95%. Combined application of macroporous resin and high-speed counter- current chromatography is simple and high efficiency for preparing fatty acid synthase inhibitors in oihea camellia extracts, which provides the basis for the exploitation and utilization of the fruit hull of oiltea camellia and further function research.
出处
《核农学报》
CAS
CSCD
北大核心
2017年第2期307-313,共7页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金(31270722)
浙江省"生物工程"重中之重学科自设课题(ZS2015004)
关键词
油茶蒲
高速逆流色谱
大孔树脂
脂肪酸合酶抑制剂
oihea camellia extracts, HSCCC, macroporous resin, fatty acid synthase inhibitor
