摘要
目的:研究金银花二氯甲烷萃取部位的化学成分。方法:采用硅胶柱层析、Sephadex LH-20柱色谱、重结晶等手段进行分离纯化,通过理化性质、MS、1H-NMR和13C-NMR等波谱学技术进行结构鉴定。结果:从金银花二氯甲烷萃取部位中分离纯化得到9个化合物,分别鉴定为:β-谷甾醇(1)、苯甲酸(2)、5-羟基-7,3',4'-三甲氧基黄酮(3)、tetrapedic acid B(4)、苜蓿素(5)、hydnocarpin D(6)、6,7,10-三羟基-8-十八烯酸(7)、5'-methoxyhydnocarpin-D(8)、胡萝卜苷(9)。结论:其中,化合物2、4、7、8为首次从该植物中分离得到,化合物2、7、8为首次从忍冬科植物中分离得到。
Objective： To study the chemical constituents of the methylene chloride extract of Lonicerae Japonicae Flos. Methods：The compounds were isolated and purified by silica gel column chromatography,Sephadex LH-20 gel column chromatography and recrystallization. The structures were elucidated by the physical and chemical properties,MS,1H-NMR and13C-NMR spectroscopy. Results： Nine compounds were isolated and their structures were identified as β-sitosterol（ 1）,benzoic acid（ 2）,5-hydroxy-7,3＇,4＇-trimethoxyflavone（ 3）,tetrapedic acid B（ 4）,tricin（ 5）,hydnocarpin D（ 6）,6,7,10-trihydroxy-8-octadecenoic acid（ 7）,5＇-methoxyhydnocarpin-D（ 8） and daucosterol（ 9）. Conclusion： Compound 2,4,7,8 are isolated from this plant,compounds 2,7,8 are isolated from Caprifoliaceae family for the first time.
出处
《中药材》
CAS
CSCD
北大核心
2016年第9期2030-2032,共3页
Journal of Chinese Medicinal Materials
基金
科技部"重大新药创制"科技重大专项(2014ZX09509001-001)
山东省中医药抗病毒协同创新中心项目(XTCX2014C01-02)
关键词
金银花
化学成分
结构鉴定
Lonicerae Japonicae Flos
Chemical constituents
Structure identification
