摘要
目的:研究山茱萸生品、制品二氯甲烷萃取部位对D-半乳糖致衰人胚肺细胞影响。方法:以人胚肺细胞为材料,通过制备D-半乳糖细胞衰老模型,以MTT、SOD、NO为指标,观察D-半乳糖对人胚肺细胞的损伤以及山茱萸生品、制品二氯甲烷萃取部位的保护作用,并通过Rt-PCR检测,进一步探讨山茱萸生品、制品二氯甲烷萃取部位对衰老通路的影响。结果:山茱萸生品、制品二氯甲烷萃取部位对D-半乳糖致衰人胚肺细胞增殖有一定的影响;对D-半乳糖致衰人胚肺细胞抗氧化系统表现出保护作用;对衰老相关通路有一定的调控作用。结论:山茱萸生品、制品二氯甲烷萃取部位对D-半乳糖致衰人胚肺细胞有一定的保护作用。
Objective：To investigate effects of dichloromethane extraction sites from raw and processed Fructus Corni on ageing human embryonic lung cells induced by D-galactose.Methods：Taking human embryonic lung cells as materials and MTT、SOD、NO as indicators,the impairment of human embryonic lung cells induced by D-galactose and protective function of dichloromethane extraction sites from raw and processed Fructus Corni were observed by making the cellular ageing model of D-galactose,and effects of dichloromethane extraction sites from raw and processed Fructus Corni on ageing pathways were further discussed by Rt-PCR test.Results：Dichloromethane extraction sites from raw and processed Fructus Corni exerted certain effect on proliferation of ageing human embryonic lung cells induced by D-galactose,showing protective effect on their antioxidant systems and having regulated effect on their pathways related to ageing.Conclusion：Dichloromethane extraction sites from raw and processed Fructus Corni have protective effects on ageing human embryonic lung cells induced by D-galactose.
出处
《辽宁中医杂志》
CAS
北大核心
2011年第12期2440-2443,共4页
Liaoning Journal of Traditional Chinese Medicine
基金
国家"十五"科技攻关计划(2001BA701A11)
国家自然科学基金(30772851)
关键词
山茱萸生品
山茱萸制品
二氯甲烷萃取部位
D-半乳糖
人胚肺细胞
raw processed Fructus Corni
processed Fructus Corni
dichloromethane extraction sites
D-galactose
human embryonic lung cells
