摘要
为了解循环水鳗鲡养殖过程中调节MBBR生物滤池的工艺后微生物群落结构与代谢功能变化情况,进而寻找能否在节能的工艺下,生物滤池中的滤料微生物仍有良好的硝化作用,通过改变生物滤池依次至O2、A/O和O/A工艺,运用Biolog-ECO法分析各工艺稳定条件下生物滤池中微生物群落的代谢特征。结果显示:AWCD值总体变化趋势上O2工艺二段明显最优,其次是A/O工艺一段,而O/A工艺一段的滤料微生物对碳源利用能力相对最差;在对六类碳源的利用情况上,不同工艺对氨基酸类和胺类代谢偏好明显,其中O2工艺二段和A/O工艺一段的滤料微生物对六类碳源利用程度相对较高;O/A工艺一段在多样性指数上处于最高水平,其次是O2工艺二段和O/A工艺二段,O2工艺一段在各多样性指数上均处于较低水平;主成分分析显示,不同工艺不同生物滤池段的滤料微生物代谢基质主成分1的贡献率为32.89%,主成分2为25.91%,主成分1相关系数0.5以上的碳源有16种,主成分2有14种;在滤料微生物对31种碳源的利用上,利用明显偏好的(>4%)有氨基酸类的L-天门冬酰胺和L-精氨酸、碳水化合物中的N-乙酰-D-葡萄糖胺、多聚物中的吐温40和吐温80、酚酸类的4-羟基苯甲酸和胺类的苯乙胺,利用率较低的为羧酸类的γ-羟基丁酸、碳水化合物的D,L-α-磷酸甘油和酚酸类的2-羟基苯甲酸。实验通过调节曝气情况改变生物滤池的工艺,研究生物滤池中的微生物群落碳代谢特征,为循环水养殖系统的进一步研究提供参考依据。
To learn the carbon metabolism of microbial communities of moving bed biofilm reactor which was adjusted to different process in recirculating aquaculture systems, and find out if microorganism had a better nitrification in moving bed biofilm reactor under the condition of energy conservation. Experiments changed the moving bed biofilm reactor to different process which wereO^2, A/O and O/A, respectively. Then, using Biolog-ECO method to analyse the characteristics of microorganism on the carbon source metabolism of moving bed biofilm reactor. The results showed that, the second section of O^2 process was the best of AWCD of microbial communities cultured in different process, then was the first section of A/O process, and the first section of O/A process was the worst. The utilization degree of carbon sources of microbial communities in different process show preference to amino acids and amines, the second section of O^2 process and the first section of A/O process were higher than the other process. The first section of O/A process was the highest in micro-organism diversity indices, then were the second section of O^2 process and the second section of O/A process, and the first section of O^2 process was the lowest. The PCA （principal component analysis） showed that contribution of PC1 of the micro-organisms in different process was 32.89% ,PC2 was 25.91% ,and there were 16 types of substrates in PC1 over 0.5 of load,and 14 in PC2. L-Asparagine and L-Arginine of amino acids, N-Acetyl-D-Glucosamine of carbohydrates, Tween 40 and Tween 80 of polymers, 4-Hydroxy Benzoic Acid of phenolic acids and Phenylethylamine of amines were better utilized by micro-organisms. 7- Hydroxybutyrie Acid of carboxylic acids, D, L-or-Glycerol Phosphate of carbohydrates and 2-Hydroxy Benzoic Acid of phenolic acids were utilized worse. This experiment through changing the work conditions of moving bed biofilm reactor to study its carbon metabolism of microbial communities, which can provide a basis for further study
作者
朱云昊
谭洪新
孙大川
罗国芝
王姣
刘颖
ZHU Yunhao TAN Hongxin SUN Dachuan LUO Guozhi WANG Jiao LIU Ying(College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China Shanghai Aquacultural Engeering Research Center, Shanghai 201306, China Shanghai Ocean University Aquatic Animal Breeding Center, Shanghai University Knowledge Service Platform, Shanghai 201306, China)
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2017年第1期64-74,共11页
Journal of Shanghai Ocean University
基金
"十二五"农村领域国家科技计划课题(2012BAD25B03)
上海市科技委员会资助项目(13320502200)
关键词
循环水养殖系统
生物滤池
Biolog-ECO
微生物群落
碳源代谢
recirculating aquacuhure system
moving bed biofilm reactor
Biolog-ECO method
microbial communities
carbon metabolism
