摘要
为了研究鳗鱼循环水养殖系统不同水处理单元微生物群落的碳代谢特征,实验采用Biolog Eco技术,分析了流化床2个槽和滴流式微珠生物过滤器上、中、下3层的生物膜微生物群落功能多样性。结果显示,流化床2个槽和滴流式微珠生物过滤器中、下层微生物多样性指数(Shannon-Wiener指数、Simpson指数和Pielou指数)无显著差异(P>0.05),但均显著高于滴流式微珠生物过滤器上层(P<0.05)。平均色度变化(AWCD)与主成分分析(PCA)均证明滴流式微珠生物过滤器上层与其他采样点微生物代谢差异较大。各采样点微生物未对ECO板某一大类碳源表现出偏好,但对衣康酸、D-半乳糖醛酸、L-精氨酸、L-天门冬酰胺、L-丝氨酸、D-甘露醇、D-木糖、N-乙酰-D-葡萄糖氨、吐温40、吐温80、苯乙胺等单一碳源利用较好;而对γ-羟丁酸、α-丁酮酸、D,L-α-磷酸甘油和1-磷酸葡萄糖利用较差。某些碳源种类如D-葡糖胺酸、α-D-乳糖、2-羟基苯甲酸仅能被部分采样点的微生物利用。本实验利用Biolog Eco PlateTM技术研究中试规模循环水处理单元微生物群落代谢特征,研究结果为生物过滤器的调控提供了一种新的思路,即可通过碳源调节来促进生物膜微生物群落结构的改变,以此提高水处理效率。
The carbon metabolism of microorganisms in biofilters of a recirculating aquaculture system（ RAS） cultured with Anguilla marmorata was investigated using Biolog Eco plates. The biofilms were collected from two different tanks of a fluidized bed filter and three different depths of a trickle filter（ top,middle and bottom）. The results showed that there is no significant difference for Shannon-Wiener index,Simpson index and Pielou index among the biofilms collected from two different tanks of fluidized bed filter and the middle and bottom of trickle filter（ P〉0. 05）,all of which was significantly higher than that in the top of the trickle filter（ P〈0. 05）. The average well color development（ AWCD） and principal component analysis all demonstrated the carbon metabolism of microorganisms in the top of trickle filter was different from other four sample sites. Microorganisms in all sample sites didn＇t showany preference for any of the six categories of carbon sources. However,the Itaconic acid,D-Galacturonic acid,L-Arginine,L-Asparagine,L-Serine,D-Mannitol,D-xylose,N-Acetyl-D-Glucosamine,Tween 40,Tween 80 and Phenylethyl-amine could be well used by microorganisms from all the sample sites,while γ-Hydroxy butyric acid,α-Ketobutyric acid,D,L-α-Glycerol Phosphate and Glucose-1-Phosphate could not be used by most of microorganisms from all the sample sites. Some carbon sources such as D-Glucosamic Acid,α-D-Lactose,and 2-Hydroxy Benzoic Acid could only be used by microorganisms from certain sample sites. The present study illustrated the properties of carbon metabolism of microorganisms in biofilter of a RAS using Biolog Eco plates and gave a newpossibility for water quality control in RAS,which will promote the microbial community changes through regulating the carbon sources in the system to manage the water quality.
出处
《水产学报》
CAS
CSCD
北大核心
2015年第10期1539-1548,共10页
Journal of Fisheries of China
基金
国家自然科学基金(31402317)
上海市优秀青年教师资助计划(ZZhy13006)
上海海洋大学博士启动基金
关键词
碳代谢
生物膜
Biolog方法
循环水养殖系统
carbon metabolism
biofilm
Biolog Eco PlateTM
recirculating aquaculture system
