摘要
目的探讨亚致死剂量球形芽孢杆菌(Bacillus sphaericus,Bs)对斯氏按蚊传疟能力的影响。方法利用亚致死剂量Bs 2 362株处理斯氏按蚊Ⅳ龄幼虫,收集存活按蚊并常规饲养。正常对照组按蚊Ⅳ龄幼虫不进行Bs处理,常规饲养。对3~5 d龄的斯氏按蚊成蚊进行约氏疟原虫攻击感染,并于按蚊吸血后第9天解剖蚊胃,观察卵囊的发育情况,比较分析Bs处理对斯氏按蚊传疟能力的影响。结果正常对照组和Bs处理组按蚊对疟原虫的感染率分别为92.1%和97.6%,Bs处理组感染率高于正常对照组,差异有统计学意义(P<0.05)。Sigma 3.5统计软件分析显示,正常对照组和Bs处理组斯氏按蚊对疟原虫的感染度分别为(56.69±69.29)和(84.19±75.64),Bs处理组按蚊卵囊数量显著高于正常对照组,差异有统计学意义(P<0.05)。结论亚致死剂量Bs 2362株处理显著增加约氏疟原虫对斯氏按蚊的感染率和感染度,提高了按蚊的传疟能力。
Objective To investigate the impact of Bacillus sphaericus（ Bs） exposure on vector competence of Anopheles stephensi to malaria parasites. Methods The 4thinstar larvae of Anophels stephensi were treated with sub-lethal dose of Bs strain 2362. The surviving pupae were collected and routinely fed. The mosquitoes of control group were maintained without Bs treatment. Mosquitoes in age of 3 ～ 5 d were challenged by infection of Plasmodium yoelii. The rate and intensity of infection were obtained through dissection of midguts on day 9 post blood feeding and observation of oocyst development. The influence of Bs treatment on capacity of anopheles vectors to transmit malaria was compared between the 2 groups. Results The infection rates of Bs treatment group and control group were 97. 6% and 92. 1%,respectively. The difference between them was statistically significant by Chi-square test（ P〈0. 05）. In addition,the infection density of Bs treatment group（ 84. 19 ± 75. 64） was higher than that of control group（ 56. 69 ± 69. 29）,which was statistically significant by Mann-Whitney test（ P〈0. 05）. Conclusion The sub-lethal dose of Bs strain2362 significantly increased the rate and intensity of Anopheles stephensi infected by Plasmodium yoelii,and consequently enhanced the vector competence of Anopheles stephensi to malaria parasites.
作者
于莎莎
付蒙颖
李秋霞
王维
王英
Yu Shasha Fu Mengying Li Qiuxia Wang Wei Wang Ying(Institute of Tropical Medicine, College of Military Preventive Medicine, Third Military Medical University, Chongqing, 400038, Chin)
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2017年第4期333-336,共4页
Journal of Third Military Medical University
基金
国家自然科学基金面上项目(81271875)
全军后勤科研"十二五"计划面上项目(CWS12J017)~~
关键词
球型芽孢杆菌
斯氏按蚊
约氏疟原虫
传疟能力
Bacillus sphaericus
Anopheles stephensi
Plasmodium yoelii
vector competence
