摘要
目的:建立伏安极谱法测定硫酸软骨素滴眼剂中硫柳汞的含量,考察不同材质的塑料滴眼剂瓶对硫柳汞的吸附情况。方法:采用多功能电极中的悬汞模式电极(HMDE),在氯化钾-盐酸缓冲溶液中加入样品溶液,氮气脱氧时间为200 s,富集时间为60s,平衡时间为5s,采用两次标准加入法,进行电位扫描测定。结果:硫柳汞的线性范围为0.01~1.0 mg·ml^(-1)(r=0.999 0),检测限为1.78μg·ml^(-1),平均回收率为99.1%(RSD=2.7%,n=9)。结论:该方法简便、准确,适用于硫酸软骨素滴眼剂中硫柳汞的含量测定。低密度聚乙烯和聚丙烯材质对硫柳汞的吸附明显,硫酸软骨素滴眼剂不宜采用上述材质包装。
Objective： To establish a volt-ampere polarography method for the determination of thimerosal in chondroitin sulfate eye drops, and study the adsorption of thimerosal in different packing materials. Methods： The hanging mercury drop electrode （ HM- DE） in multi-functional electrode was used and the sample solution was added to potassium chloride - hydrochloric acid buffer solution. Nitrogen deoxygenation time was 200 s, the enrichment time was 60 s, and the equilibrium time was 5 s. The potentiometric scan was performed using a two standard addition method. Results： Thimerosal had a good linear relationship within the range of 0.01-1.0 mg ml-l（r =0. 9990）. The detection limit was 1.78 μg m1-1 and the average recovery was 99.1% （RSD = 2.7%, n = 9）. Conclusion： The method is simple and accurate, and suitable for the determination of thimerosal in chondroitin sulfate eye drops. Low-density polyethylene and polypropylene both show obvious adsorption of thimerosal, so chondroitin sulfate eye drops should not be packed in the above packing materials.
作者
初玎鲒
姜红
胡敏
Chu Dingjie Jiang Hong Hu Min(Hubei University of Chinese Medicine, Wuhan 430075, China Hubei Institute for Food and Drug Control)
出处
《中国药师》
CAS
2017年第2期380-382,共3页
China Pharmacist
关键词
硫酸软骨素滴眼剂
硫柳汞
含量测定
伏安极谱法
Chondroitin sulfate eye drops
Thimerosal
Content determination
Volt-ampere polarography method
