摘要
以商麦5226种子为试验材料,采取模拟干旱和盐分交叉胁迫小麦幼苗的方法,通过测定交叉胁迫下小麦种子的发芽率、发芽势、可溶性糖含量、丙二醛(MDA)含量、可溶性蛋白含量等指标,以探讨小麦随盐旱胁迫时间变化渗透调节物质的变化规律,为培育抗盐抗旱的优良小麦品种提供理论基础。结果表明,小麦种子的发芽率、发芽势随着盐旱胁迫程度的增加呈逐渐下降的趋势;在0.6%盐溶液和10%的聚乙二醇交叉胁迫条件下,小麦幼苗具有一定的交叉适应性,发芽率为83%,发芽势为96%,可溶性糖在第2天达到最大值(2.56 mg/g),与对照相比增加了14.29%,降低了细胞水势,维持细胞正常生长代谢;丙二醛缓慢上升再下降,消除活性氧对植物的破坏,提高植物的抗氧化能力;可溶性蛋白在第2天达到最大值(0.34 mg/g),并分解成各种氨基酸,尤其是脯氨酸的含量增多,降低水势,减轻盐旱所造成的伤害。
The paper took Shangmai 5226 seeds as experimental material,adopted the simulation method of intercross stress of drought and salt on wheat seedlings,under the intercross stress by measuring the wheat seed germination rate,germination potential,soluble sugar content,malondialdehyde（MDA） content,soluble protein content and other indicators,to explore the change rule of osmotic regulation substances of wheat with salt drought stress time,to provide theoretical basis for the cultivating of excellent wheat varieties of salt and drought resistance.The results showed that the germination rate and germination potential of wheat seeds decreased gradually with the increase of salt and drought stress.In 0.6%NaCI solution concentration,with 10%PEG stress had certain cross adaptation,germination rate was 83%,the germination potential was 96%,soluble sugar in 2 days reached maximum,and compared to the control increased by 0.32 rng/g,decreased the cell water potential,maintain the normal growth and metabolism of cells,malondialdehyde slowly increased and then decreased,eliminating the damage of reactive oxygen species of plants,improving the antioxidant capacity of plants,soluble protein in the second day reached the maximum0.34 mg/g,and decomposition into various arrino acids,especially prdine content increased,decreased water potential,reduced the salt and drought caused by damage.
出处
《山西农业科学》
2017年第2期166-171,共6页
Journal of Shanxi Agricultural Sciences
基金
陕西省科技厅农业攻关项目(2011K 01-18)
关键词
小麦
盐旱交叉胁迫
渗透调节
wheat
salt and drought cross stress
osmotic adjustment
