摘要
为了进一步研究Ta EF-1α基因在小麦响应干旱胁迫中的作用,首先在正常灌水和干旱胁迫2个处理下对Ta EF-1α基因在转录水平上的表达量进行了验证,并利用VIGS(Virus induced gene silencing)技术将Ta EF-1α基因进行沉默,对其功能进行研究。结果表明,Ta EF-1α基因在m RNA水平上的表达量与其蛋白水平上的变化趋势一致,均受干旱诱导上调表达。与正常灌水且未进行VIGS沉默处理的植株相比,干旱处理且未进行VIGS沉默处理的植株中Ta EF-1α基因表达量极显著升高,其叶片相对含水量也极显著降低、相对电导率和丙二醛(MDA)含量则极显著升高;干旱条件下,与未进行VIGS沉默的对照植株相比,VIGS沉默后的植株中Ta EF-1α基因表达量极显著下降,其叶片相对含水量也极显著降低、相对电导率和丙二醛(MDA)含量则极显著升高,植株表现出叶片下垂、对干旱胁迫敏感的表型。综合干旱处理和VIGS沉默Ta EF-1α基因后小麦植株的表型和生理指标变化,说明干旱胁迫诱导小麦植株中Ta EF-1α基因上调表达,敲低Ta EF-1α基因表达后小麦抗旱性显著下降,因此,Ta EF-1α基因在小麦响应干旱胁迫中可能发挥了重要作用。
The expression level of the protein product of TaEF-1αgene was up-regulated under drought stress condition in one of our previous study using iTRAQ technology.To further study the function of TaEF-1αgene in response to drought stress in wheat,we quantified the expression of TaEF-1αgene at the transcriptional level gene under normal and drought stress conditions,the expression level of TaEF-1αgene was knock down by using virus induced gene silencing(VIGS)technique to study its function.The results showed that the expression of TaEF-1αgene was up-regulated by drought stress.Under drought stress condition,the expression level of TaEF-1αgene and the relative water content of leaves decreased significantly and the relative conductivity and malondialdehyde(MDA)content increased significantly in knock-down-plants than wild type plants.The changes in phenotypes and physiological indices of wheat plants after drought stress and VIGS silencing TaEF-1αgene indicated that the drought-induced TaEF-1αgene expression in wheat plants was up-regulated,and the TaEF-1αgene knock-down-plants also exhibited leaf drooping earlier and more sensitive to drought stress than wild type plants.Taken together,TaEF-1αgene may play a crucial role in response to drought stress in wheat.
作者
王新博
任永哲
岳慧芳
李乐
吕伟增
史校艳
辛泽毓
王志强
林同保
WANG Xinbo;REN Yongzhe;YUE Huifang;LI Le;LV Weizeng;SHI Xiaoyan;XIN Zeyu;WANG Zhiqiang;LIN Tongbao(Agricultural College,Henan Agricultural University,Zhengzhou 450002,China;Collaborative Innovation Center of Henan Grain Crops,Zhengzhou 450002,China;State Key Laboratory of Wheat and Maize Crop Science Provincial Department of Construction,Zhengzhou 450002,China)
出处
《华北农学报》
CSCD
北大核心
2018年第2期72-78,共7页
Acta Agriculturae Boreali-Sinica
基金
国家重点研发计划项目(2016YFD0300205)
河南省自然科学基金项目(162300410133)
