摘要
目的:研究舒血宁注射液辅助治疗对慢性阻塞性肺疾病(COPD)急性加重期患者Toll样受体(TLRs)所介导炎症反应的影响。方法:选择2014年5月~2015年12月期间在我院接受舒血宁注射液联合常规西医药物治疗的COPD急性加重期患者进行研究,治疗前和治疗后1、2、3、4周时采集外周血单个核细胞并测定TLRs表达量,采集血清并测定炎性因子、气道重塑分子的含量。结果:治疗后1、2、3、4周时,COPD患者外周血单个核细胞中TLR2、TLR4的mRNA表达量以及血清中肿瘤坏死因子(TNF)-α、白介素(IL)-6、单核细胞趋化因子-1(MCP-1)、血管细胞黏附分子-1(VCAM-1)、基质金属蛋白酶(MMP)2、MMP9、转化生长因子(TGF)-β、骨形成蛋白(BMP)-2的含量均显著低于治疗前,TLR3、TLR7、TLR8、TLR9的mRNA表达量与治疗前比较无显著性差异;外周血单个核细胞中TLR2、TLR4的mRNA表达量与血清中TNF-α、IL-6、MCP-1、VCAM-1、MMP2、MMP9、TGF-β、BMP-2的含量呈正相关关系。结论:舒血宁注射液辅助治疗对COPD急性加重期患者TLRs所介导炎症反应具有显著抑制效应。
Objective：To study the effect of adjuvant Shuxuening injection therapy on the inflammatory response mediated by TLRs in patients with acute exacerbation of COPD.Methods：Patients with acute exacerbation of COPD who received Shuxuening injection combined with conventional western medicine treatment in our hospital between May 2014 and December2015were selected for study,and before treatment as well as 1week,2weeks,3weeks and 4weeks after treatment,peripheral blood mononuclear cells were collected to determine TLRs expression,and serum was collected to determine the levels of inflammatory cytokines and airway remodeling molecules.Results：At 1week,2weeks,3weeks and 4weeks after treatment,TLR2and TLR4 mRNA expression in peripheral blood mononuclear cells as well as TNF-α,IL-6,MCP-1,VCAM-1,MMP2,MMP9,TGF-βand BMP-2levels in serum of patients with COPD were significantly lower than those before treatment,and TLR3,TLR7,TLR8 and TLR9 mRNA expression were not significantly different from those before treatment;TLR2and TLR4 mRNA expression in peripheral blood mononuclear cells were positively correlated with serum TNF-α,IL-6,MCP-1,VCAM-1,MMP2,MMP9,TGF-βand BMP-2levels.Conclusions：Adjuvant Shuxuening injection therapy has significant inhibitory effect on the inflammatory response mediated by TLRs in patients with acute exacerbation of COPD.
作者
李杰
徐敏
LI Jie XU Min(Department of Respiratory Medicine, Gong＇an County People＇s Hospital in Hubei Province, Jingzhou City 434300, Hubei Prov- ince, China ICU, Gong＇an Hospital of Traditional Chinese Medicine in Hubei Province, Jingzhou City 434300, Hubei Province, China)
出处
《海南医学院学报》
CAS
2017年第4期471-474,共4页
Journal of Hainan Medical University
基金
荆州市科技局科研项目(2012-14-11)~~
关键词
慢性阻塞性肺疾病
舒血宁
Toll样受体
炎性因子
气道重塑
Chronic obstructive pulmonary disease
Shuxuening
Toll-like receptor
Inflammatory cytokines
Airway remodeling
