摘要
在超声波辅助和80℃下,以无水乙醇为溶剂对南方红豆杉进行提取,浓缩后得提取物EE,EE通过柱色谱法分离制得紫杉醇,并对EE和紫杉醇的抗氧化、抗肿瘤活性进行研究。结果表明,EE对DPPH·、ABTS^+·、O_2^-·、OH·的清除活性显著优于常用抗氧化剂BHT和紫杉醇,半清除浓度IC_(50)分别为13.19±1.50、2.65±0.35、25.33±1.75和343.00±2.13mg/L,说明EE具有优良的抗氧化活性;紫杉醇对人非小肺癌细胞A549、人乳腺癌细胞MCF-7、人肝癌细胞Bel-7402和子宫颈癌细胞Hela的抑制活性远优于EE,半抑制浓度IC_(50)分别为3.45±1.37、6.20±0.88、1.98±0.49和1.15±0.27mg/L。
Under ultrasound,Taxus chinensis var.mairei were extracted with ethanol at 80 ℃ to offer ethanol extract（EE）.EE was further extracted with chloroform and separated by column chromatography to offer taxol.Their antioxidant and antitumor activities were then evaluated.The antioxidant activity screening results showed that EE exhibited better scavenging activity than the common antioxidant BHT in these four assays,with IC50 of 13.19±1.50,2.65±0.35,25.33±1.75and343.00±2.13 mg/L,respectively,while taxol showed the worst,indicating excellent antioxidant activity of EE.The antitumor activity screening results demonstrated that taxol showed better inhibition than EE,with IC50 of 3.45±1.37,6.20±0.88,1.98±0.49 and 1.15±0.27mg/L,respectively.
出处
《广西师范大学学报（自然科学版）》
CAS
北大核心
2016年第4期55-59,共5页
Journal of Guangxi Normal University:Natural Science Edition
基金
国家自然科学基金资助项目(21501032)
广西自然科学基金资助项目(2014GXNSFBA118050
2016JJA120113)
广西高等学校优秀中青年骨干教师培养工程资助项目(桂教办[2013]258号)
广西高校中青年教师基础能力提升资助项目(KY2016YB441)
梧州学院重点科研项目(2014B011)
广西植物功能物质研究与利用重点实验室开放基金资助项目(FPRU2013-6)
关键词
南方红豆杉
紫杉醇
抗氧化活性
抗肿瘤活性
超声波提取
Taxus chinensis var.mairei
taxol
antioxidant activity
antitumor activity
ultrasound-assisted extract
