摘要
通过RT-PCR和RACE技术,从荔枝叶片中克隆得到了一条SAE2基因的全长c DNA序列,命名为Lc SAE2。Lc SAE2全长为2 348 bp,编码643个氨基酸。Lc SAE2为带负电荷的稳定亲水蛋白,没有信号肽和跨膜结构域。进化树分析表明,Lc SAE2与葡萄和蓖麻植物中的SAE2亲缘关系较近。q PCR结果表明,Lc SAE2在"元红"荔枝同组织部位中均有表达,在新叶与芽中表达量较高,在焦核与正常核之间表达量差异明显,可能参与荔枝芽、叶生长及胚胎发育的调控。
In this study, a full-length c DNA sequence of SUMO-activating enzyme 2 gene（ SAE2） was obtained from leaves of Litchi chinensis cv. Yuanhong using RT-PCR and RACE, named Lc SAE2.The complete c DNA sequence of Lc SAE2 was 2 348 bp, encoding 643 amino acids. Lc SAE2 was stable and hydrophilic protein, without signal peptides and trans-membrane domain structures.Phylogenetic tree analyses indicated that Lc SAE2 had a close genetic relationship with Vitis vinifera and Ricinus communis. The results of Real Time PCR indicated that the Lc SAE2 expressed in all the tested tissues, and it expressed at the highest level in the new leaves and buds, the difference of expression levels of Lc SAE2 between aborted-seeds and normal seeds significant. It was concluded that it might be participate in bud and leaf growth, as well as in embryonic development.
作者
吕科良
程春振
陈裕坤
林玉玲
郑诚乐
陈桂信
赖钟雄
LV Ke-liang(College of Horticulture/Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fujian,Fuzhou 35000)
出处
《园艺与种苗》
CAS
2016年第11期35-38,共4页
Horticulture & Seed
基金
福建省科技重大专项(2013NZ0002)
