摘要
目的探究纳米二氧化钛对小鼠成纤维（Balb/3T3）细胞的毒性作用。方法将处于对数生长期的Balb/3T3细胞暴露于含终浓度分别为0（对照）、1、10、50、100μg/ml纳米二氧化钛颗粒（粒径分别为30、50、100 nm）的DMEM完全培养基中培养24 h。采用CCK-8法测定细胞活性,通过测定细胞培养液上清中LDH的活力来检测细胞膜的完整性,并通过测定细胞内ROS含量来探讨细胞毒性的产生机制。结果与对照组比较,10~100μg/ml不同粒径的纳米二氧化钛染毒组Balb/c3T3细胞的存活率均较低,差异有统计学意义（P〈0.05）;且随着纳米二氧化钛染毒浓度的升高和纳米二氧化钛粒径的下降,Balb/c3T3细胞的存活率呈下降趋势。与对照组比较,10~100μg/ml不同粒径的纳米二氧化钛染毒组Balb/c3T3细胞培养液上清中LDH的活力和ROS的含量均较高,差异有统计学意义（P〈0.05）;且随着纳米二氧化钛染毒浓度的升高,Balb/c3T3细胞培养液上清中LDH的活力和ROS的含量均呈上升趋势。结论纳米二氧化钛对小鼠成纤维细胞的毒性作用呈明显的剂量效应和尺寸效应,纳米二氧化钛诱导Balb/c3T3细胞产生大量活性氧（ROS）可能是导致其产生细胞毒性的主要原因。
Objective To investigate the cytotoxic effect of nano-TiO_2 with different concentrations and sizes on mice fibroblasts. Methods Mice fibroblasts Balb/c3T3 were cultured in vitro and nano-TiO_2 with different concentrations（0,1,10,50,100 μg/ml） and sizes（30 nm,50 nm,100 nm） were added into the medium. After 24 hours of treatment, the cell activity was evaluated by CCK-8 method, the cell membrane integrity was detected by cell culture supernatant of LDH activity,the mechanism of cytotoxicity was analyzed by ROS level. Results The viability of Balb/c3T3 cells, which were treated with different sized nano-TiO_2, decreased significantly compared with the control group（P〈0.05）;With the increase of nano-TiO_2 concentrations and the declines of sizes, the viability of Balb/c3T3 cells decreased（P〈0.05）, the activity of LDH and the ROS content presented arising in cell culture supernatant after treatment with different sizes and concentrations of nano-TiO_2（P〈0.05）. Conclusion Cytotoxicity of nano-TiO_2 to Balb/c3T3 cells is related to its concentration and size. Increasing of ROS induced by Nano-TiO_2 may be one important element of cytotoxicity.
出处
《环境与健康杂志》
CAS
北大核心
2016年第10期864-866,共3页
Journal of Environment and Health
基金
黑龙江省自然科学基金(H201369)
佳木斯大学重点科研项目(Sz2013-001)
