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响应面法优化胡芦巴多糖超声提取工艺及体外抗氧化活性的研究 被引量:4

Study on Ultrasonic-assisted Extraction Process of Total Polysaccharide from Trigonella Foenum-graecum and Its Antioxidant Activity in Vitro
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摘要 以胡芦巴为原料,采用超声波法提胡芦巴多糖.根据单因素实验的结果,选定液料比、超声功率、超声时间、提取温度四个因素为变量,按照Box-Behnken试验设计原理,以多糖提取率为考查指标,进行响应面设计,利用SAS软件对实验结果分析,得到自变量与多糖提取率之间的回归方程.通过对体外清除DPPH·、·OH、O_(2-)·能力的测定,考察胡芦巴多糖的体外抗氧化活性.实验结果显示胡芦巴多糖的最佳提取工艺参数为:液料比27 1(m L/g),超声功率240 W、超声时间107 min,提取温度68℃.在最佳工艺参数条件下胡芦巴多糖提取率的预测值为21.79%,实际提取率为21.28%,与理论值间偏差为-2.34%.胡芦巴多糖具有一定的抗氧化活性,并且对DPPH·、·OH、O_(2-)·的清除率均呈现浓度依赖性,对三种自由基半数清除浓度(EC50)分别为:0.93、0.51、0.30 mg/m L. Polysaccharide was extracted by ultrasonic assisted water method with Trigonella foenum-graecum as raw material.On the basis of single factor experiment, the liquid material ratio, ultrasonic power, ultrasonic time and extraction temperature were chosen as independent variables. According to the principle of Box-Behnken design, response surface design was performed with polysaccharide extraction rate as the indexes. Result analysis was performed using SAS software and the second order quadratic equation between the variables and polysaccharide extraction rate was obtained. Furthermore, the antioxidant activity of Polysaccharides from Trigonella foenum-graecum was studied through determination of the ability of scavenging free radicals, i. e. DPPH · , · OH, O2- · . Finally, the experimental results showed that the optimum extraction process of Polysaccharide from Trigonella foenum-graecum are as follows : liquid to solid ratio 27 : 1 ( mL/g) , ultrasonic power 240 W,ultrasonic time 107 min,and extraction temperature 68℃.Under these conditions,the extraction rate of polysaccharide was 21.79% and the actual extraction rate was 21.28%. The deviation between the theoretical value and the theoretical value was -2.34%.The polysaccharide from Trigonella foenum-graecum has certain antioxidant activity and the scavenging rate of DPPH· , ·OH and O2-· showed a dependent man-ner of concentration.And the half clearance rates (EC50) were 0.93,0.51 and 0.30 mg/mL,respectively.
出处 《吉林化工学院学报》 CAS 2017年第1期8-15,共8页 Journal of Jilin Institute of Chemical Technology
关键词 胡芦巴 多糖 超声波辅助提取 抗氧化 Trigonella foenum-graecum polysaccharide ultrasonic-assisted extraction antioxidant
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