摘要
目的探讨遵义人群载脂蛋白A5（ApoA5）基因c．553G／T位点多态性与混合型高脂血症的相关性。方法收集222名遵义地区人群静脉血标本，其中混合型高脂血症组100例，正常对照组122名，用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术检测两组标本ApoA5基因c．553G／T位点多态性，分析混合型高脂血症组和正常对照组中基因型频率和基因频率分布规律，及其与混合型高脂血症的关系。结果ApoA5 c．553G／T位点基因型频率和基因频率在混合型高脂血症组与正常对照组差异有统计学意义（χ^2=12．081，P=0．001；χ^2=17．469，P〈0．001）；通过Logistic回归校正年龄、性别、血糖后，T等位基因携带者（TT＋GT基因型）患高脂血症的风险较GG基因型携带者增加（OR=6．042，95％CI：1．962—18．607，P=0．002）。结论ApoA5 c．553G／T位点多态性与遵义地区人群混合型高脂血症发病存在一定相关性，ApoA5 c．553T等位基因可能是混合型高脂血症的独立危险因素。
Objective To investigate the relationship between c. 553G/T polymorphism of apolipoprotein A5 （ApoAS） gene and combined hyperlipidemia in Zunyi population. Methods Blood samples from 222 subjects including 100 patients with combined hyperlipidemia and 122 healthy individuals in Zunyi area were collected, c. 553G/T polymorphism was tested by polymerase chain reaction and restriction fragment length polymorphism （PCR-RFLP） assay in both groups. The distribution of the genotypes and allele frequencies in the combined hyperlipidemia group and normal control group were analyzed, and its relation- ship with combined hyperlipidemia was analyzed. Results The genotypes and gene frequencies of the ApoA5 gene c. 553G/T showed statistical difference between combined hyperlipidemia group and normal control group （χ^2 = 12.081, P = 0. 001 ; χ^2 = 17. 469, P 〈 0.001 ）. The risk of hyperlipidemia for carriers of T alleles （TF or GT genotypes） was higher than those of GG genotypes （OR =6. 042, 95% CI： 1. 962- 18.607, P=0.002）, after adjusting age, gender and blood glucose by Logistic regression analysis. Conclusions ApoA5 gene c. 553G/T polymorphisms has a relationship with combined hyperlipidemia in Zunyi population. It may be an independent risk factor for combined hyperlipidemia.
出处
《国际内分泌代谢杂志》
2017年第1期6-10,F0003,共6页
International Journal of Endocrinology and Metabolism
基金
贵州省科技厅联合基金资助项目（黔科合J字LKZ[2012]39号）
遵义医学院大学生创新创业训练计划项目（院发[2014]5828）
