摘要
目的 :测定不同工艺提取的龙血竭中紫檀的含量。方法 :以C18反相键合硅胶为固定相 ,乙腈 1%冰醋酸 (41∶5 9)为流动相 ,检测波长为 319nm ,用外标法定量。结果 :紫檀在 10 .4～ 10 4ng范围有良好线性关系 ,r =0 .9992 ,方法回收率为 98.76 % ,RSD为 1.74 %。新型的免加热提取工艺的龙血竭中紫檀的含量低于加热提取工艺龙血竭中紫檀的含量。结论 :免加热提取龙血竭明显优于加热提取工艺。
Objective: To establish HPLC method for determination of pterostilbene in Dragon's Blood with different extraction technology. Method: Applying a C 18 phase column and acetonitrile 1% acetic acid(41∶59) as mobile phase, detecting at 319nm and quantitating with external standard method. Results: The standard curves of pterostilbene was linear in the concentration range of 10.4～104ng, r =0.9992. The average recovery was 98.28%, RSD =1.97%. The content of pterostilbene in Dragon's Blood extracted with ordinary temperature was lower than that with heating technology. Conclusion: The content of pterostilbene extracted with ordinary temperature extraction in Dragon's blood have the advantage of heating extraction.
出处
《中成药》
CAS
CSCD
北大核心
2002年第8期616-618,共3页
Chinese Traditional Patent Medicine
基金
国家自然科学基金资助项目 (3 0 0 70 0 88)
