摘要
目的建立大鼠肺纤维化模型,并对其进行评价。方法经气管内喷雾注入博莱霉素(Bleomycin,BLM)致大鼠肺纤维化,于第1、2、3、4周处死大鼠,观察大鼠给药后日常状况和体重变化,测定羟脯氨酸(L-hydroxyproline-trans-4-hydroxy-L-proline,HYP)含量、肺重,计算肺系数,取肺组织制备病理切片,进行HE、Masson染色后,评价肺组织形态学变化。结果给药4周后,模型组大鼠肺部产生了明显肺纤维化症状,肺系数及HYP含量均明显高于对照组,肺泡间隔增厚,纤维化区可见大量蓝色胶原纤维沉积。结论大鼠气管内喷雾注入BLM可成功制备肺纤维化模型,且纤维化分布较均匀。
Objective To establish and evaluate the rat model of pulmonary fibrosis. Methods Rat model of pulmonary fibrosis was established by tracheal spraying with bleomycin（BLM）. Partial rats were killed at weeks 1,2,3 and 4 after treatment with BLM respectively,observed for daily status and body weight and determined for L-hydroxyproline（HYP）content and lung weight,based on which the pulmonary coefficient was calculated. The lung tissues of the rats were prepared into sections,and subjected to HE and Masson staining to evaluate the morphological change. Results Obvious fibrosis was observed in the lungs of rats 4 weeks after treatment with BLM,while the pulmonary coefficient and HYP content were significantly higher than those in control group. However,the pulmonary alveolar septals of model rats were thickened,and a large quantity of blue collagen fibers was deposited in the fibrosis area. Conclusion The rat model of pulmonary fibrosis was established successfully by tracheal spraying with BLM,and the fibrosis was distributed evenly.
作者
彭方毅
周欢
吴静
姜海蓉
PENG Fang-yi ZHOU Huan WU Jing JIANG Hai-rong(Traditional Chinese Medicine Hospital of Dianjiang County, Chongqing City, Chongqing 408300, Chin)
出处
《中国生物制品学杂志》
CAS
CSCD
2017年第1期86-89,共4页
Chinese Journal of Biologicals
基金
国家自然科学基金资助项目(30571714
60873103)
国家自然科学基金重点项目(30830090)
重庆市科委自然基金(CSTC
2008BB5331
2009BB7231)
教育部科技重点项目(210178)
