摘要
目的研究丙型肝炎患者血清白细胞介素32(IL-32)的表达水平及与丙型肝炎病毒(HCV)载量的相关性,探讨IL-32在丙型肝炎中的发病机制。方法 70例丙型肝炎患者血清中IL-32的表达水平采用ELISA检测,并检测丙型肝炎患者血清丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST),用Roche荧光定量PCR系统检测血清HCV载量,同时测定40例健康对照者血清中IL-32和ALT、AST表达水平。结果丙型肝炎患者的血清IL-32、ALT、AST水平显著高于健康对照组(P<0.01),呈正相关关系;丙型肝炎患者血清中IL-32水平与血清HCV载量呈正相关关系。结论 IL-32与丙型肝炎的发病密切相关,可能是因为HCV能够促进IL-32的合成与释放。
Objective To study the relationship between serum interleukin 32（IL-32）expression and hepatitis C virus（HCV）load in patients with hepatitis C,and to explore the role of IL-32 in the pathogenesis of HCV.Methods The expression level of IL-32 in 70cases of patients with hepatitis C in serum was detected by enzyme-linked immunosorbent assay（ELISA）,and the serum alanine aminotransferase（ALT）and aspartate aminotransferase（AST）were detected.Serum HCV viral load was detected by Roche fluorescence quantitative PCR system,and the expression levels of IL-32,ALT and AST were measured in 40 healthy control subjects.Results The levels of IL-32,ALT and AST in serum of patients with hepatitis C were significantly higher than those in healthy control group（P〈0.01）,and showed a positive correlation;there was a positive correlation between serum IL-32 level and serum HCV load in patients with hepatitis C.Conclusion IL-32 is closely related to the pathogenesis of hepatitis C,probably because HCV can promote the synthesis and release of IL-32.
出处
《检验医学与临床》
CAS
2017年第2期184-186,189,共4页
Laboratory Medicine and Clinic
基金
国家自然科学基金资助项目(81102-002)
