摘要
目的探讨杨桃根总提取物(EACR)对糖尿病小鼠肾损伤及其抗氧化应激的作用。方法小鼠尾静脉注射120mg·g^(-1)链脲佐菌素(STZ)建立糖尿病模型小鼠,随机分为5组:模型对照组、缬沙坦组(20 mg·kg^(-1))、EACR低、中、高剂量组(300、600、1 200 mg·kg^(-1)),再设正常对照组,每组10只小鼠。分别于药前、药后测各组小鼠空腹血糖(FBG)。末次给药后,收集血清和尿液,检测小鼠血清中肌酐(Cr)和尿素氮(BUN)的含量以及24 h尿量及尿蛋白水平;试剂盒检测小鼠肾脏组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性和丙二醛(MDA)含量;HE染色观察肾脏组织的病理学变化;ELISA法检测肾组织中氧化氢酶(CAT)、活性氧簇(ROS)的含量;蛋白免疫印迹法检测肾组织中Cyto-C、AIF、caspase-3的蛋白表达。结果与模型组比较,缬沙坦组以及EACR中、高剂量组的血清生化指标和24h尿蛋白水平明显降低,差异具有统计学意义(P<0.05)。糖尿病小鼠给药后,EACR能明显降低MDA含量,提高SOD、GSH-Px、CAT活性;下调ROS、Cyto-C、AIF、caspase-3的表达(P<0.05)。HE结果显示EACR还能改善糖尿病小鼠肾小球病理变化。结论杨桃根总提取物可减轻糖尿病小鼠血清中Cr和BUN的水平,降低肾脏组织中MDA、ROS含量,以及提高组织中抗氧化因子SOD、GSH-Px活性及CAT的含量,下调肾组织中Cyto-C、AIF、caspase-3的蛋白表达,缓解氧化应激对肾组织所造成的损伤,从而起到改善糖尿病小鼠肾损伤的作用。
Aim To investigate the effect of the extract of Averrhoacarambola L. root（ EACR） on renal function in diabetic mice and its anti-oxidative action.Methods Diabetic mice were established by tail vein injection with 120 mg·kg^（-1）streptozotocin（ STZ） and were divided into 5 groups： model control group,valsartan control group,and low-,middle-,high-dose of EACR groups（ 300,600,1 200 mg·kg^（-1））. And10 normal mice consisted of normal control group. The fasting blood glucose（ FBG） of mice was detected before and after administration of drugs. After last administration,the blood and urine samples were collected for creatinine（ Cr）,urea nitrogen（ BUN）,urine and24 h urinary protein determination. The activities of superoxide dismutase（ SOD）,glutathione peroxidase（ GSH-Px） and malonaldehyde（ MDA） content were determined using kits. HE staining was conducted to observe the pathological changes of kidney tissues.ELISA method was utilized to detect the contents of catalase（ CAT） and reactive oxygen species（ ROS）.The expressions of Cyto-C,AIF and caspase-3 proteins in kidney tissues were analyzed by Western blot. Results Compared with model group,the serum biochemical indexes and 24 h urinary protein of valsartan and moderate-,high-dose of EACR groups were decreased with statistical significance（ P 0. 05）. After the treatment, the MDA content was decreased by EACR treatment,and SOD,GSH-Px and CAT activities were enhanced. Meanwhile the expressions of ROS,Cyto-C,AIF and caspase-3 were down-regulated. Thepathological changes of kidney tissues were ameliorated by EACR through HE results. Conclusions The extract of Averrhoacarambola L. root can decrease the serum levels of Cr and BUN,reduce the MDA and ROS contents in kidney tissue and enhance the activities of SOD,GSH-Px and CAT,down-regulate the expressions of Cyto-C,AIF and caspase-3 proteins in kidneytissues,elevate the anti-oxidative effect of kidney.Therefore,the renal function of diabetic mice is meliorated.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2017年第1期95-100,共6页
Chinese Pharmacological Bulletin
基金
广西研究生教育创新计划资助项目(No YCBZ2015035)
国家自然科学基金资助项目(No 81360129
81460205)
南宁市青秀区科学研究与技术开发计划(No 2013S12)
关键词
杨桃根总提取物
糖尿病
肾损伤
氧化应激
ROS
促凋亡因子
extract of Averrhoacarambola L.root
diabetes
renal injury
oxidative stress
ROS
apoptogenic factor
