摘要
目的探讨过敏性休克豚鼠多种器官TLR4的表达,为过敏性休克死亡的法医学鉴定寻找新指标。方法将35只豚鼠随机分为实验组(26只)和对照组(9只),实验组豚鼠注射人血清致敏原建立过敏性休克豚鼠模型,对照组注射生理盐水。采用RT-PCR及免疫组织化学分别检测2组豚鼠死后即时、冷藏48h、冰冻7d气管、肺、胃和脾等组织TLR4的表达水平。结果实验组豚鼠死后即时、冷藏48h、冰冻7d气管、肺、胃和脾TLR4mRNA及蛋白表达水平均较对照组显著升高(P<0.05);实验组豚鼠死后各时段气管、肺、胃和脾TLR4mRNA及蛋白表达水平比较差异均无统计学意义(P>0.05)。结论 RT-PCR、免疫组织化学检测气管、肺、胃及脾TLR4表达可能为法医学鉴定过敏性休克猝死提供参考;过敏性休克死亡在尸体保存条件良好情况下,TLR4在7d内降解不明显。
Objective To detect the expression of TLR4 in various organs in guinea pigs with anaphylactic shock,and to find a new indicator for forensic identification of lethal anaphylactic shock.Methods Thirty-five guinea pigs were randomly divided into two groups.The experimental group（n=26）were injected with human serum allergens to establish the model of anaphylactic shock.The control group（n=9）only received normal saline.The expression of TLR4 in trachea,lung,stomach and spleen were measured by RT-PCR and immunohistochemistry（IHC）immediately after death,after cold storage for 48 hours and after frozen storage for 7days.Results Compared with control group,the expression of TLR4 mRNA and protein in trachea,lung,stomach and spleen increased in experimental group at all time points（P〈0.05）.However,no significant differences in TLR4 expression were found among different time points in experimental group（P〉0.05）.Conclusion RT-PCR and IHC measurement of TLR4 expression in trachea,lung,stomach and spleen may assist in the diagnosis of lethal anaphylactic shock.The degradation of TLR is not obvious within 7days in corpses in a good state of preservation.
出处
《南昌大学学报（医学版）》
CAS
2016年第6期4-8,共5页
Journal of Nanchang University：Medical Sciences
基金
江西省自然科学基金(20132BAB205061)
