摘要
目的复制过敏性休克豚鼠动物模型,探讨IL-4与IL-10在豚鼠血清中的表达,为法医学和临床对过敏性休克的诊断提供科学、客观的参考依据。方法采用多人混合血清建立过敏性休克的豚鼠模型,豚鼠40只(实验组30只,对照组10只),用酶联免疫吸附试验(ELISA)检测过敏性休克豚鼠血清中IL-4与IL-10的表达情况。结果实验组血清IL-4、IL-10水平明显高于对照组(P<0.01)。结论实验组血清IL-4与IL-10水平明显高于对照组,为过敏性休克的法医学和临床的诊断提供了参考依据。
Objective To establish animal model of guinea pig anaphylactic shock ,explore the expression of IL‐4 and IL‐10 in guinea pig serum ,provide scientific and objective evidence for medicolegal and clinical diagnosis of anaphylactic shock as well .Meth‐ods After 40 guinea pigs were classified into the experimental and the control groups ,in which the experimental group was 30 and the control group was 10 ,Elisa method was used to detect IL‐4 and IL‐10 by extracting serum of guinea pigs in the experiment group and the control group .Results The IL‐4 levels of serum in experimental group was higher than that in control group （P〈0 .01）;the IL‐10 levels of serum in experimental group was higher than that in control group （P〈0 .01） .Conclusion The level of serum IL‐4 and IL‐10 in the experiment group are obviously higher than that of the control group .This provides an evidence for an‐aphylactic shock forensic identification and clinical diagnosis .
出处
《重庆医学》
CAS
CSCD
北大核心
2014年第15期1898-1900,共3页
Chongqing medicine
基金
贵州省科学技术基金资助项目(黔科合J字2012-2362)
