摘要
目的探讨不同抗原修复方法在陈旧性石蜡切片乳腺珠蛋白免疫组化中的效果。方法选取2010年1月至2012年4月本实验室保存的陈旧组织石蜡切片样品76例,所有样品组织均取自乳腺癌患者,各取连续3张切片分别归入试验组、高压组和微波组。微波组和高压组按照常规加热修复法处理相应患者石蜡切片,试验组在pH为3.5±0.1的柠檬酸盐缓冲液中室温修复15min。比较三组切片的抗原修复效果。结果三组切片经过不同的处理方法修复后,肿瘤细胞染色结果显示,试验组染色强度及高阳性比例分布优于高压组和微波组,差异具有统计学意义（χ~2=11.122、14.468、12.859、13.267,P〈0.05）。综合阳性标记分数显示,试验组阳性分数多为＋＋、＋＋＋,与高压组、微波组分布比较差异有统计学意义（χ~2=13.713、10.331,P〈0.01）。试验组阳性率明显高于后两组,差异具有统计学意义（χ~2=6.199、4.475,P〈0.05）。试验组经修复后的脱片率（χ~2=22.800、27.059,P〈0.01）及折损率（χ~2=64.083、40.827,P〈0.01）均明显低于高压组、微波组。结论常温下pH为3.5±0.1柠檬酸盐缓冲液进行抗原修复,能减少组织脱片及折损的发生率,提高切片修复效果。
Objective To discuss the effects of different antigen retrieval methods in mammaglobin immunohistochemistry with old paraffin sections. Methods 76 old paraffin sections of tissue preserved in the laboratory from June 2012 to May 2013 were selected, and three consecutive slices of each section were divided into the experiment group, high pressure group or microwave group respectively. The microwave group and high pressure group were treated according to the conventional heating retrieval method, while the experi- ment group was treated at room temperature in pH 3.5 citrate buffer for 10 to 15 minutes. The results of antigen retrieval in three groups were compared. Results The experiment group was superior to the high pressure group and the microwave group in terms of strong positive section and high proportion （χ2 = 11. 122, 14. 468, 12. 859, 13. 267, P〈0.05） . Most of the positive scores in the experiment group were ＋＋ or ＋＋＋, significantly different from those in the microwave group and high pressure group（ χ2 = 13. 713, 10. 331, P〈0.01） . The positive rate in the experiment group was significantly higher than that in the oth- er two groups （χ2 =6. 199, 4. 475, P〈0.05） . In the test group the unfailing rate after restoring and the discount ratio were all significantly lower than those in hypertension group and microwave group （χ2 = 64. 083, 40. 827, P〈0. 01） . Conclusion Antigen retrieval with pH 3.5 citrate buffer at normal temperature can reduce the incidence of tissue dehydration and deterioration, and improve the effect of retrieval.
出处
《中国微生态学杂志》
CAS
CSCD
2016年第12期1429-1432,共4页
Chinese Journal of Microecology
