摘要
目的检测乳腺癌及乳腺良性病变患者外周血中人乳腺珠蛋白(human mammary gland globin,h MAM)m RNA的表达,为乳腺癌分子标记选择提供理论依据。方法选取2011年6月至2012年10月期间笔者所在医院收治的乳腺癌患者78例以及乳腺良性病变患者30例(乳腺增生症15例,乳腺纤维腺瘤15例)作为研究对象,应用聚合酶链反应(PCR)技术检测其外周血中h MAM m RNA的表达,并分析其与患者年龄、肿瘤大小、病理学类型、肿瘤分期、腋窝淋巴结转移以及ER、PR和HER-2表达的关系。结果乳腺增生及乳腺纤维腺瘤患者外周血中均未检出h MAM m RNA表达,乳腺癌患者外周血中h MAM m RNA表达阳性率为48.72%(38/78)其差异有统计学意义(χ2=12.357,P=0.000)。乳腺癌患者外周血中h MAM m RNA的表达与患者年龄、肿瘤大小、病理学类型以及ER、PR和HER-2表达无关(P>0.05),但与肿瘤的临床分期(Z=-2.214,P=0.027)和淋巴结转移相关(Z=-2.754,P=0.006)。结论外周血中检出的h MAM m RNA表达是诊断乳腺癌相对特异性的标志,外周血中h MAM m RNA的表达可能与乳腺癌患者的预后相关。
Objective To test the expressions of human mammary gland globin （hMAM） mRNA in the peripheral blood of breast cancer and breast benign lesions patients, try to provide the theory basis for the choice of breast cancer molecular marker. Methods Polymerase chain reaction （PCR） technology was used to detect the expressions of hMAM mRNA in peripheral blood of 78 cases of breast cancer patients, 15 cases of hyperplasia of mammary gland, and 15 cases of breast fibroadenoma. The relationship between the expressions of hMAM-mRNA in peripheral blood of breast cancer patients with patient＇s age, tumor size, pathological type, tumor stage, axillary lymph node metastasis, and the ER, PR and HER-2 status were analyzed. Results The expressions ofhMAM-mRNA in peripheral blood were not detected in breast hyperplasia and breast fibroadenoma patients, but the peripheral blood hMAM-mRNA expression rate in breast cancer patients was 48.72% （38/78）, the difference was statistically significant （X2=12.357, P=0.000）. The expression of peripheral blood hMAM mRNA was not related to the patient＇s age, tumor size, pathological type, and ER, PR and HER-2 status （P〉0.05）, but the expression of peripheral blood hMAM mRNA was related to the clinical staging of tumor （Z=-2.214, P=0.027） and lymph node metastasis status （Z=-2.754, P=0.006）. Conclusion Peripheral blood hMAM- mRNA detected is a sign of breast cancer, further research is needed to confirm whether hMAM mRNA detection in peripheral blood correlates with poor prognosis of breast cancer patients.
出处
《中国普外基础与临床杂志》
CAS
2015年第1期91-95,共5页
Chinese Journal of Bases and Clinics In General Surgery
基金
湖北省十堰市科技局指导项目(项目编号:2010 st04)~~
关键词
乳腺癌
人乳腺珠蛋白
MRNA
Breast cancer
Human mammary gland globin
mRNA
