摘要
目的研究转化生长因子(TGF)α-435G/A基因多态性与非酒精性脂肪性肝病(NAFLD)发病之间的相互关系。方法选取我院2013年2月至2015年2月收治的NAFLD患者126例作为研究对象,同时以121例正常人作为对照组,采用限制性片段长度多态性(RFLP)分析法对上述研究对象外周血TGFα-435G/A基因进行多态性分析,所获得的数据采用SPSS软件进行分析。结果 NAFLD患者TGFα-435G/A基因型与等位基因的分布与对照组之间并无显著性差异,但是NAFLD患者中AA和GG基因型频率(39.2%和16.3%)和等位基因频率(61.4%和38.6%)与对照组之间(分别为25.7%和35.3%,和45.2%和54.8%)存在显著性差异(x^2=15.43,P<0.05;x^2=16.32,P<0.05);进一步的研究表明,不同程度NAFLD患者AA、AG、GG基因型频率和等位基因频率也存在一定的差异,即重度脂肪肝患者纯合体(AA和GG)比例相对较高。结论 TGFα-435G/A多肽位点基因突变与NAFLD患者发病之间存在一定的关联性,且不同基因型频率和等位基因频率与病情存在一定的相关性。
Objective To study the transforming growth factor α（TGF-α）-435G/A gene polymorphism in patients with nonalcoholic fatty liver diseases（NAFLD）. Methods 126 patients with NAFLD between February 2013 and February 2015 were recruited in our hospital,and 121 healthy individuals were also included at the same time as control. The polymorphism of TGF-α -435G/A gene in peripheral blood was analyzed by restriction fragment length polymorphism （RFLP） and the data obtained were processed by SPSS software. Results There was no significant difference neither in the TGFα -435G/A genotypes nor the distribution of TGFα-435G/A allele between patients with NAFLD and control（P〉0.05）,however,there were significant differences in AA and GG genotype frequency （39.2% vs. 25.7%,and 16.3% vs. 35.3%） and their allele frequency （61.4% vs. 45.2%,and 38.6% vs. 54.8%） between the two groups （x^2=15.43,P〈0.05;x^2=16.32,P〈0.05）;And further study showed that patients with NAFLD with different steatosis had different AA GG,AG genotype frequencies and their allele frequencies with relatively high percentage of homozygote（AA,GG） in patients with severe fatty liver diseases. Conclusions There is a certain correlation between the gene mutation of TGFα-435G/A and the incidence of NAFLD,and the frequency of different genotypes and allele might be related to the severity of NAFLD.
出处
《实用肝脏病杂志》
CAS
2017年第1期65-68,共4页
Journal of Practical Hepatology
