摘要
目的建立杏花药材的质量控制方法。方法以绿原酸、芦丁为对照品,建立薄层色谱以鉴别药材。采用紫外波长转换检测的高效液相色谱法同时测定药材中阿魏酸、芦丁及异槲皮苷的含量,色谱柱为YMCPack ODS-A C18柱(250mm×4.6mm,5μm),流动相为甲醇-乙腈-0.1%磷酸溶液(125∶105∶770);阿魏酸的检测波长为322nm(0~25 min),芦丁与异槲皮苷的检测波长为255nm(25~70 min);流速:1.0mL/min;柱温:30℃。结果薄层色谱中斑点清晰;阿魏酸、芦丁及异槲皮苷分别在1.452 2~14.521 7μg/mL(r=0.998 9)、27.366 7~273.666 8μg/mL(r=0.997 2)、1.649 9~16.499 0μg/mL(r=0.999 6)范围内线性关系良好,平均加样回收率分别为99.86%、99.70%、100.03%;RSD分别为0.9%、0.8%、1.2%。结论所用方法准确、稳定、可行,可用于杏花药材的质量控制。
Objective To establish the quality control method for the medicinal material apricot flower. Methods Thin layer chromatography （TLC） was performed with chlorogenic acid and rutin as references for the identification of medicinal material. High-performance liquid chromatography （HPLC） with ultra- violet wavelength switching detection was used to measure the content of ferulic acid, rutin, and isoquercitrin in the medicinal material. HPLC was performed on a YMC-Pack ODS-A C18 column （250 mm × 4.6 mm, 5 μm） with a mobile phase of methanol-acetonitrile-0.1% phosphoric acid （125： 105： 770） at a column temperature of 30 ℃, a flow rate of 1.0 mL/min, and a wavelength of 322 nm （0-25 min） for ferulic acid and 255 nm （25-70 min） for rutin and isoquercitrin. Results The TLC spots were clear. Ferulic acid, rutin, and isoquercitrin showed a good linear relationship within the ranges of 1. 452 2-14. 521 7 μg/mL （r=0. 998 9）, 27. 366 7-273. 666 8 μg/mL （r=0. 997 2）, and 1. 649 9-16. 499 0 μg/mL （r=0. 999 6）, respectively, with average recovery rates of 99.86% （RSD=0.9%）, 99. 70% （RSD=0. 8%）, and 100.03 % （RSD= 1.2%）, respectively. Conclusion The method is accurate, stable, and feasible and can be used for quality control of apricot flower.
出处
《安徽中医药大学学报》
2017年第1期73-76,共4页
Journal of Anhui University of Chinese Medicine
关键词
杏花
薄层色谱法
高效液相色谱法
阿魏酸
芦丁
异槲皮苷
Apricot flower
Thin layer chromatography
High-performance liquid chromatography
Ferulic acid~
Rutin
Isoquercitrin
