摘要
运用中压柱色谱和高效制备液相色谱方法对多穗金粟兰的化学成分进行分离纯化,利用现代波谱技术鉴定分离得到的化合物结构,并对其进行体外细胞毒活性测试.从多穗金粟兰乙醇提取物中分离得到8个单体化合物,分别鉴定为迷迭香酸(1)、迷迭香酸甲酯(2)、isorinic acid(3)、isorinic acid methyl ester(4)、(E)-N-苯乙基-3,4-(亚甲二氧基)丙烯酰胺(5)、咖啡酸(6)、oresbiusin A(7)、5-羟基尿嘧啶(8),化合物3~6、8均为首次从该植物中分得.采用MTT法对化合物1~8进行体外细胞毒活性测试,结果显示化合物2、4、8对2个肝癌细胞株HepG2和Hep3B均有抑制作用,它们的IC_(50)值范围为116.8~150.8μmol/L.
The chemical constituents of the traditional medicine ＂Sikuaiwa＂ （ Chloranthus multistachys Pei） were isolated and purified with medium-pressure column chromatography and preparative HPLC. These isolated chemical constituents were identified with modern spectroscopic techniques and assayed with vitro cytotoxicity. Eight pure compounds were obtained and their structures were elucidated as rosmarinic acid （ 1 ）, rosmarinic acid methyl ester （ 2）, isorinic acid （ 3 ）, isorinic acid methyl ester （4） , （E） -N-Phenethyl- 3,4- （ methylenedioxy ） cinnamamide （ 5 ）, caffeic acid （ 6）, oresbiusin A （7） and 5-hydroxyuracil （8） based on detailed spectral anal- ysis.Compounds 3--6 and 8 were isolated from the title plant for the first time.In vitro cytotoxic activity of compounds 1--8 were evaluated by using MTT method,and the results showed that compound 2,4 and 8 exhibited cytotoxic activity against HepG2 and Hep3B cell lines with IC50 values ranging from 116.8 to 150.8μmol/L.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
北大核心
2017年第1期124-129,共6页
Journal of Yunnan University(Natural Sciences Edition)
基金
国家自然科学基金(81573561
81102798)
青海省自然科学基金(2016-ZJ-908)
关键词
多穗金粟兰
细胞毒活性
化学成分
Chloranthus multistachys Pei
cytotoxicity
chemical constituents
