摘要
目的研究全反式维甲酸(ATRA)与顺铂对人头颈癌TCA8113细胞凋亡的影响。方法 ATRA和顺铂单独及联合作用于TCA8113细胞,采用MTT法检测细胞生长,并观察细胞形态变化;使用流式细胞仪检测细胞周期及凋亡;通过western blot实验检测caspase-3表达情况。结果 ATRA作用于TCA8113细胞后,细胞生长明显受到抑制,细胞形态发生改变,细胞凋亡比例明显增加,caspase-3蛋白表达下调,ATRA与顺铂联合作用于肿瘤细胞后,其抑癌作用加强。结论 ATRA联合顺铂对人头颈癌TCA8113细胞具有协同抑癌作用。
Objective To observe the effect of all-trans retinoic acid（ATRA）and cisplatin on the proliferation of human head and neck cancer TCA8113 cells. Methods TCA8113 cells were treated with ATRA and（or）cispl-atin respectively. Cell proliferation was detected by the method of MTT. Cell cycle was detected by flow cytometry. Results The growth of the cells treated with ATRA was inhibited obviously and the morphology of the cells were changed. The apoptosis rate of TCA8113 cells was increased gradually and the effect was enhanced when combined ATRA with cisplatin. Conclusion ATRA could inhibit the proliferation of human head and neck cancer TCA8113 cells and the inhibitory effect was synergistic when ATRA combined with cisplatin.
出处
《肿瘤基础与临床》
2016年第4期281-283,共3页
journal of basic and clinical oncology
基金
国家自然科学基金资助项目(编号:81402231)
河南省科技厅基础研究和前沿技术研究项目(编号:142300410315)
郑州市口腔颌面外科院士工作站资助项目(编号:152PYSGZ040)
郑州大学第一附属医院院内青年基金项目
