摘要
建立了酶解法（葡聚糖酶、β-葡萄糖苷酶、葡萄糖氧化酶和过氧化氢酶）测定小麦籽粒中的β-葡聚糖含量,对该方法的标品测定回收率和加标回收率进行研究,利用该方法测定了8个品种小麦籽粒中β-葡聚糖含量,并分析其含量的差异性。结果表明：酶解法具有良好的测定结果;8个品种小麦籽粒中β-葡聚糖含量在0.44%~0.68%之间,小麦籽粒中β-葡聚糖含量由于品种和产地的不同而呈现一定的差异性,试验结果为从膳食中摄入β-葡萄糖提供了参考。
The Enzymatic Hydrolysis Method including dextranase,β-glucosidase,glucose oxidase and catalase was established for the determination on the content of β-glucan in wheat grain. The standard determination recovery rate and sample added recovery rate of the method were investigated and the differences of the β-glucan contents from 8 varieties of wheat grain were compared. The results showed that the Enzymatic Hydrolysis Method had good determination results,and the β-glucan content of 8 varieties of wheat grain were between 0. 44%and 0. 68%. Differencs were found in the β-glucan content among different varieties. This results provided new insight into the consumption ofβ-glucan from diet.
作者
白沙沙
赵萌
于佳立
陈月晓
崔亚娟
BAI Sha-sha ZHAO Meng YU Jia-li CHEN Yue-xiao CUI Ya-juan(Beijing Research Institute for Nutritional Resources, Beijing 100069, China Beijing City University, Beijing 100094, China)
出处
《中国食物与营养》
2016年第12期69-72,共4页
Food and Nutrition in China
基金
北京市级财政项目"食物中四类植物化学物检测技术的开发改进及植物化学物配方食品加工方法研究"(项目编号:PXM2014_178102_000011)
关键词
小麦
Β-葡聚糖
酶解法
wheat
β-glucan
Enzymatic Hydrolysis Method
