摘要
本研究分析了不同缺水条件下(缺水0 h,缺水2 h和缺水10 h)两个大豆品种(抗旱品种DT2008和栽培品种Williams82)的差异表达基因,并对差异表达基因进行了功能和通路富集分析。进一步通过整合大豆相关miRNA数据库,挖掘了miRNA与差异表达基因的调控网络。结果表明,在缺水2 h条件下抗旱品种DT2008表达相对较多的基因,差异基因数是Williams82的1.87倍;随着缺水时间由2 h增加到10 h,DT2008差异表达基因数没有显著变化,而Williams82差异表达基因增加了311个,表明干旱胁迫对于DT2008基因表达影响相对较小。差异表达基因主要富集到了apoptosis、cell death、response to stimulus及binding等生物学过程和分子功能,这些基因的差异表达降低了干旱胁迫诱导下的细胞凋亡并为干旱胁迫下大豆的生长发育提供了必需的营养。miRNA和mRNA调控网络分析结果表明,miR166家族、miR2118a-5p和miR2118b-5p能够调节Glyma13g23680.1和Glyma02g10320.1基因表达并参与大豆抗旱胁迫应答。
In this study, the differentially expressed genes （DEGs） of drought-tolerant DT2008 and cultivar under normal （0 h treatment） and dehydration conditions （2 h treatment and 10 h treatment） were explored. Then DEGs were subjected to function and pathway enrichment analysis. Further, regulation networks between soybean related miRNAs and DEGs were constructed. Results showed that more DEGs were identified in DT2008 after 2 h dehydration treatment and the number was 1.87 times of Williams82. There was no significant change in the number of DEGs in DT2008 when dehydration condition was extended to 10 h. But the DEGs in Williams82 increased by 311. This results indicated that dehydration had little influence on DT2008. Function and pathway enrichment analysis showed that DEGs mainly enriched in apoptosis, cell death, response to stimulus and bind and et al. Hence, those DEGs probably reduced cell apoptosis and provided nutrition, miRNA and mRNA interaction network analysis indicated that miR166 family, miR2118a-5p and miR2118b-5p can regulate Glyma13g23680.1 and Glyma02g10320.1 gene expressions and participated in drought-resistance.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2016年第12期3514-3520,共7页
Genomics and Applied Biology
基金
山西省自然基金项目(2014011030-2)
农业部转基因生物新品种培育重大专项(2009ZX08003-005B)
农业部转基因专项(2013ZX08004002)共同资助
山西省财政支农项目(015zzcx-23)
山西省种业发展专项项目(2014ZYFZ-11)
国家高技术研究发展计划(863计划)重点项目(2007AA021403)
关键词
大豆
抗旱
差异表达基因
miRNA
Soybean, Drought resistance, Differentially expressed genes, miRNA
