摘要
该文首先将多壁碳纳米管（MWCNTs）滴涂在玻碳电极（GCE）的表面;然后用循环伏安法和电沉积的方法依次沉积对氨基苯酚（AP）和纳米金（Au）,最后制成金/聚对氨基苯酚/多壁碳纳米管纳米复合材料修饰电极（Au/p-AP/MWCNT/GCE）。采用循环伏安法和示差脉冲伏安法等电化学技术研究了尿酸（UA）在该修饰电极上的电化学行为。结果表明该修饰电极对UA具有较好的电催化活性,在pH6.0磷酸盐缓冲溶液中,其检测下限为3.3μmol/L,线性范围分别为15-510μmol/L。干扰实验表明,共存物质抗坏血酸（AA）和多巴胺（DA）对UA的检测没有干扰。同时该修饰电极用于实际样品（尿液和血清）中UA的测定也得到较好的效果。该研究为UA的快速检测提供了一种新方法。
In this paper, firstly, the multi-walled carbon nanotubes （MWCNTs） were coated on the surface of glassy carbon electrode （GCE）; then, p-aminopheno （ALP） and gold nanoparticles （Au） were deposited by cyclic vohammetry and electrodeposition; finally, an Au/poly （p-aminopheno）/multi-walledcarbon nanotubes （Au/p-AP/MWCNTs） nano-composite materials modified electrode was fabricated. The electrochemical behavior of UA was investigated by cyclic voltammogram and differential pulse voltammetry, indicating that the modified electrode possessed an enhanced electrocatalytic activity to the UA.In 0.1 moUL PBS （pH6.0）, the modified electrode showed good response to UA, the linear calibrations for UA were obtained over ranges of 15 to 510 μmol/L with detection limits of 3.3 μmol/L. The interference test showed that the coexisted ascorbic acid （AA） and dopamine （DA） had no electrochemical interference toward UA. In addition, the modified electrode was successfully applied for the determination of analytes in real sample （urine and serum） with satisfactory results. This study provides a new method for the rapid detection of UA.
作者
王存
王益平
彭莉
张毅
Wang Cun Wang Yi-ping Peng Li Zhang Yi(Department of Biological ＆ Chemical Engineering, Chongqing University of Education, Chongqing 400067, China Chongqing food and drug inspection and Testing Research Institute, Chongqing Engineering Research Center for Pharmaceutical Process and Quality Control, Chongqing 401121, China)
出处
《化学传感器》
CAS
2016年第3期37-42,共6页
Chemical Sensors
基金
重庆市教委科学技术研究项目(KJ1501404)
重庆市工程技术研究中心建设项目(cstc2015yfpt-gcjsyjzx0027)资助
关键词
多壁碳纳米管
纳米金
聚对氨基苯酚
尿酸
玻碳电极
multi-walled carbon nanotubes
gold nanoparticles
poly （p-aminopheno）
uric acid
glassy carbon electrode
