摘要
为提升烟梗品质,降低烟梗中大分子蛋白质、果胶和纤维素含量,发展了烟梗的酸性蛋白酶-果胶酶-纤维素酶的复合酶水解方法,并采用全局搜索方法对复合酶的水解条件进行了优化和验证。结果表明:不同酶对不同参数的响应不同,3个酶对p H均很敏感,尤以酸性蛋白酶最为敏感,作用区间较窄,果胶酶和纤维素酶对温度较为敏感,酸性蛋白酶受温度影响较小。整体上,3个酶在限定范围内达到最优水解率时的作用参数均不相同;参数优化后的复合酶对烟梗中的大分子物质具有较好的水解效果,总水解效率达28.34%,且感官品质较好;复合酶处理后的烟梗,其品质提升较为明显;结合工业生产特点,感官品质需求,及烟梗中蛋白、果胶和纤维素含量特点,可以有目的的在限定条件下优化烟梗水解效率及方向。
The study aimed to optimize the hydrolysis parameters for mixed enzymes to improve the quality of tobacco stems and reduce the contents of macromolecules protein,pectin and cellulose. Results showed that acid protease was more sensitive to pH than pectease and cellulase but much less affected by temperature. The optimized parameters resulted in the better degradation of macromolecules with a total hydrolysis efficiency of 28.34% and significantly improved the sensory quality of tobacco stems. These indicated that hydrolysis rate and direction of tobacco stems could be changed according to industrial manufacturing, the requirement of sensory quality and the contents of macromolecules.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2016年第11期1206-1211,共6页
Journal of Food Science and Biotechnology
基金
云南中烟工业有限责任公司科技项目(20146CP03
2013GY02)
关键词
复合酶
参数优化
烟梗
水解率
mixed-enzyme ,parameter optimization, tobacco stems, hydrolysis rate
