摘要
目的：：监测卵巢癌患者外周血中游离干细胞因子（ SCF）的浓度变化和c-kit蛋白在卵巢癌组织中的表达,探讨外周血SCF值对早期预测c-kit （＋）上皮性卵巢癌继发性化疗耐药的临床价值。方法：利用量子点双染免疫荧光（ QDs）检测106例卵巢癌组织中c-kit和SCF蛋白表达,ELISA法检测46例上述卵巢癌患者化疗期间冰冻保存的外周血SCF值,分析铂类为主的化疗药物敏感型和耐药型卵巢癌患者外周血SCF值与组织c-kit表达的差异。结果：继发性化疗耐药的上皮性卵巢癌组织中,c-kit（＋）表达率远高于化疗敏感组（29.27％ vs 10.8％,P＜0.05）,c-kit（＋）表达率随着FIGO分期的进展而升高（Ⅰ~Ⅱ期8.7％,Ⅲ~Ⅳ期25％,P＜0.05）。继发性化疗耐药组的外周血SCF值随铂类为主的化疗药物治疗而有升高的趋势,化疗敏感组的SCF值随之有下降的趋势；继发性化疗耐药组的外周血SCF均值高于化疗敏感组（ P＜0.05）。卵巢癌c-kit （＋）的外周血SCF值均值高于c-kit（-）组（P=0.018）,外周血SCF值在卵巢癌c-kit（＋）组随铂类为主的化疗耐药而升高。结论：卵巢上皮癌组织中c-kit蛋白阳性表达可能导致肿瘤对铂类化疗药物耐药；c-kit蛋白在肿瘤间质细胞内的阳性表达,预示卵巢上皮性癌化疗耐药微环境的形成和极差的临床结局。卵巢癌化疗期间,监测外周血SCF值升高可能是早期预测c-kit（＋）卵巢癌继发性化疗耐药的标记物之一。
Objective：To monitor the changes in the concentration of free stem cell fac-tor （ SCF） in peripheral blood and the expression of c-kit protein in ovarian cancer tissues,and to investigate the clinical value of early prediction of c-kit （＋） in epithelial ovarian cancer （EOC） resistance to platinum-based chemotherapy by monitoring SCF in peripheral blood. Methods：Expressions of c-kit and SCF were retrospectively studied with quantum dots immuno-fluorescence histochemistry （ QDs-IHC） ,in paraffin-embeded tissue specimens from 106 cases of EOC. The plasma concentration of the SCF were measured with enzyme linked immuno-sor-bent assay （ ELISA） from 46 EOC. To investigate the difference of SCF value in peripheral ser-um and c-kit expression of EOC patients who were sensitive and resistant to platinum-based chemotherapy. Results：In epithelial ovarian cancer （ EOC） ,the positive expression rate of c-kit in the chemoresistance group was 29. 27%,it was statistically higher than chemosensitive group （10. 8%）（P〈0. 05）. The positive expression of c-kit in EOC was closely corralated with ad-vanced FIGO clinical stages and chemotherapy-sensitivity. The positive rate increased in ad-vanced stage （Ⅰ~Ⅱ8 . 7%,Ⅲ~Ⅳ25%） （ P〈0 . 05 ） . The mean plasma concentration of the SCF in the chemoresistance group was statistically higher than the chemosensitive group of EOC （ P〈0 . 05 ） . The plasma concentration of the SCF in the chemoresistance group was up-regula-ted after platinum-based chemotherapy. The mean value of the SCF in c-kit （＋） group was higher than that in c-kit （-） group in epithelial ovarian cancer（P=0. 018）,the plasma con-centration of the SCF in c-kit （＋） group was up-regulated after platinum-based chemotherapy. Conclusion：The abnormal expression of c-kit protein may play an important role in the progres-sion and chemoresistance of EOC. The positive expression of c-kit protein in ovarian cancer stromal cell indicated poor clinical outcome
作者
彭光彩
王晓雯
易村犍
Peng Guangcai Wang Xiaowen Yi Cunjian(Department of Obstetrics and Gynecology, The First Affiliated Hospital of Yangtze University,Jingzhou 43430)
出处
《现代妇产科进展》
CSCD
北大核心
2016年第12期891-894,899,共5页
Progress in Obstetrics and Gynecology
