摘要
目的 探讨周细胞覆盖率及淋巴管密度在鼻息肉组织中的特点及意义.方法 选取26例鼻息肉患者的息肉组织（鼻息肉组）及下鼻甲组织（下鼻甲组）和11名健康对照组的鼻黏膜组织（对照组）,通过免疫组化方法检测各组织中白蛋白的表达量,同时用淋巴管特异性标志物（D2-40）检测组织中的微淋巴管密度;通过血管内皮细胞标志物（CD34）和周细胞标志物（α-SMA）免疫荧光双染,检测各组中的微血管密度和周细胞覆盖情况,并计算周细胞覆盖率.采用SPSS 17.0软件对数据进行统计学分析.结果 鼻息肉组的白蛋白含量为0.269±0.096,明显高于下鼻甲组的0.159±0.078和对照组的0.138±0.045,差异有统计学意义（q值分别为4.873、4.446,P值均＜0.05）.鼻息肉组的微血管密度为30.52±4.42,略高于下鼻甲组的30.33±6.03和对照组的28.21±6.84,但差异均无统计学意义（q值分别为0.130、1.147,P值均＞0.05）.鼻息肉组的周细胞覆盖率为0.291±0.096,明显低于下鼻甲组的0.432±0.101和对照组的0.416±0.071,差异有统计学意义（q值分别为5.399、3.680,P值均＜0.05）.鼻息肉组的淋巴管密度为0.245±0.073,明显低于下鼻甲组的0.431±0.054和对照组的0.470±0.078,差异有统计学意义（q值分别为10.004、9.328,P值均＜0.05）.鼻息肉组织中的白蛋白含量与周细胞覆盖率呈负相关（r=-0.889,P ＜0.05）,与微淋巴管密度呈负相关（r=-0.901,P ＜0.05）.结论 鼻息肉组织微血管密度正常,但存在微血管周细胞覆盖率降低且微淋巴管密度降低的微循环障碍,该特点可能与鼻息肉的发生有关.
Objective To investigate the role of lymph vessel density （LVD） and microvessel pericyte coverage index （MPI） in the pathogenesis of nasal polyps.Methods Using immunohistochemistry and immunofluorescence double staining method,the expressions of albumin,D2-40 and CD34-α-SMA in 11 specimens of normal nasal mucosa,26 specimens of nasal polyp and 26 specimens of inferior turbinate tissue from patients with nasal polyps were investigated.The counts of microvessel density （MVD）,lymph vessel density （LVD） and microvessel pericyte coverage index （MPI） were compared.SPSS 17.0 software was used to analyze the data.Results The nasal polyp group（0.269 ±0.096） had more albumin than inferior turbinate tissue group （0.159 ± 0.078） and normal nasal mucosa group （0.138 ± 0.045）,the differences were significant （q value was 4.873,4.446,both P 〈 0.05）.The counts of MVD in nasal polyp group （30.52 ± 4.42） were not significantly higher than those in inferior turbinate tissue group （30.33 ± 6.03） and normal nasal mucosa group（28.21 ±6.84）,the differences were not significant （q value was 0.130,1.147,both P 〉0.05）.The MPI in nasal polyp group （0.291 ±0.096） was significantly lower than those in inferior turbinate tissue group（0.432 ± 0.101） and normal nasal mucosa group（0.416 ± 0.071）,the difference was significant （q value was 5.399,3.680,both P 〈 0.05）.The counts of LVD in the nasal polyp group （0.245 ± 0.073） were significantly lower than those in inferior turbinate tissue group （0.431 ± 0.054） and normal nasal mucosa group （0.470 ± 0.078）,the difference was significant （q value was 10.004,9.328,both P 〈 0.05）.MPI expression in the nasal polyp group was negetively correlated to albumin expression（r =-0.889,P 〈 0.05）.The LVD expression in the nasal polyp group was negetively correlated to albumin expression（r =-0.901,P 〈 0.05）.Conclusion Different LVD and MIP in nasal polyp tissues and normal nasal mucosa tissues impl
作者
崔娜
朱冬冬
关桂梅
沙骥超
孟粹达
李琳
修倩
Cui Na Zhu Dongdong Guan Guimei Sha Jichao Meng Cuida Li Lin Xiu Qian(Department of Otorhinolaryngology Head and Neck Surgery, China-Japan Unite Hospital of Jilin University, Changchun 130033, China)
出处
《中华耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2016年第12期923-928,共6页
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
基金
基金项目：十二五国家科技支撑计划课题（2014BA107804）
国家自然科学基金（81170892、81371075、81500775）
吉林省自然科学基金（20160101118JC）
关键词
鼻息肉
微循环
毛细血管
淋巴管
周细胞
Nasal polyps
Microcirculation
Capillaries
Lymphatic vessels
Pericytes microvessel
