摘要
以木聚糖为唯一碳源,采用平板筛选和摇瓶发酵相结合的方法,从腐朽木头中分离、筛选到一株高产木聚糖酶菌株XE-13-1根据菌株的形态特征,并结合18S rDNA序列分析,初步鉴定菌株X E-13-1为桔绿木霉(Trichoderma citrinoviride)。经测定,菌株X E-13-1所产木聚糖酶的最适反应温度为50℃,最适反应pH值7,具有较好的pH稳定性。在30℃、pH 5~10的条件下保温2 h,该酶仍具有78%以上的酶活力。Mg^(2+)、Fe^(2+)、K^+、Zn^(2+)对该酶具有明显的促进作用,Cu^(2+)对该酶具有明显的抑制作用。酶学性质研究结果表明,该酶在工业应用上具有较好的前景。
Using xylan as unique only carbon source, a high xylanase-producing strain XE-13-1 was screened from the rotten wood by plate screening and shake-flask fermentation method. According to the strain morphological characteristics and 18S rDNA sequence analysis, the strain XE-13-1 was identified as Trichoderma citrinoviride. The optimal reaction temperature and pH of xylanase produced by strain XE-13-1 were 50 ℃ and 7, respectively. The xylanase had good pH stability. The xylanase activity was more than 78% after preservation for 2 h at 30 ℃ and pH 5-10. Mg2＋, Fe2＋, K＋ and Zn2＋ had obvious promoting effect on the xylanase, however, Cu2＋ had obvious inhibitory effect on the xylanase. The results of enzymatic properties indicated that the xylanase had good prospect in industrial application.
作者
马焕
权淑静
刘德海
陈国参
陶尧堂
解复红
MA Huan QUAN Shujing LIU Dehai CHEN Guocan TAO Yaotang XIE Fuhong(Henan Engineering Research Center of Industn＇al Enzyme, Zhengzhou 450008, China Henan Academy of Sciences Institute of Biology Co., Ltd., Zhengzhou 450008, China Hubei Yingcheng Yihe Central School, Yingcheng 432416, China)
出处
《中国酿造》
CAS
北大核心
2016年第12期123-128,共6页
China Brewing
基金
2014年河南省中国科学院科技成果转移转化中心项目(2014308)
2015年河南省基金项目(154200510025)
2016年河南省预先研究项目
关键词
木聚糖酶
桔绿木霉
酶学性质
xylanase Trichoderma citrinoviride enzymatic properties
