摘要
目的构建鼠疫耶尔森菌F1抗原蛋白的大肠杆菌分泌表达载体。方法 PCR法扩增鼠疫耶尔森菌的F1抗原基因,并插入大肠杆菌分泌表达载体p BAD/gⅢC中构建分泌表达载体。结果扩增出了长约500 bp的鼠疫耶尔森菌F1抗原基因并构建了该抗原的大肠杆菌分泌表达载体。结论本研究中我们用载体p BAD/gⅢC构建鼠疫耶尔森菌F1抗原的大肠杆菌分泌表达质粒,为后期制备针对鼠疫F1抗原的单克隆抗体或多克隆抗体,进一步建立便捷高效的鼠疫诊断方法奠定基础。
Objective To construct a E.coli secretory expression vector of antigen F1 of Yersinia pestis.Methods The antigen F1 was amplified by the use of PCR.The F1 gene was sub- cloned into the multiple clone sites of plasmid p BAD/gⅢ C to construct E.coli secretory expression vector.Results The sequence of 500 bp of F1 was amplified from Yesinia pestis and the E.coli secretory expression vector was constructed.Conclusion In the present study,we cloned the coding sequence of F1 capsular antigen of the bacteria in the p BAD/g III C plasmid for later expression and purification of the protein to produce poly and monoclonal antibodies against this antigen,and subsequently to develop rapid and efficient diagnostics tools for Y.pestis infection.
作者
王铁峰
王照
吴丹
刘冬冬
WANG Tie-feng WANG Zhao WU Dan LIU Dong-dong(Baicheng Normal University, Jilin 137000 ,China)
出处
《中国地方病防治》
CAS
北大核心
2016年第9期974-975,共2页
Chinese Journal of Control of Endemic Diseases
基金
吉林省卫生厅科研课题项目(2012Z065)
