摘要
目的探讨复方氯己定对种植体周围炎病原菌牙龈卟啉单胞菌菌斑生物膜的影响。方法在钛片上建立牙龈卟啉单胞菌菌斑生物膜,通过扫描电镜观测其生长状况以及加入复方氯己定后的生长状况;激光共聚焦显微镜观察加入复方氯己定对生物膜膜层厚度的影响;采用结晶紫法检测复方氯己定对牙龈卟啉单胞菌菌斑生物膜的影响。结果扫描电镜显示加入复方氯己定后种植体周围炎病原菌未能形成良好的生物膜,激光共聚焦显示加入复方氯己定后生物膜整体为红色荧光,说明出现了较多死菌,氯己定破坏了生物膜的完整性;结晶紫法测得复方氯己定组OD值(0.206±0.007),与阴性对照组(0.351±0.005)相比,显著下降,差异具有统计学意义(P<0.05)。结论作为口腔常见含漱液之一的复方氯己定对牙龈卟啉单胞菌菌菌斑生物膜的形成有一定程度的抑制作用。
Objective To study the effect of compound chlorhexidine on the biofilm of the pathogen of o-ral peri-implantitis.Methods We established plaque biofilm of the pathogen on the surface of titanium and observed the growth condition between and after adding compound chlorhexidine by scanning electron mi-croscope （ SEM） .The effects of compound chlorhexidine on the biofilm of Porphyromonas gingivalis were in-vestigated using crystal violet method.CLSM was used to observe activity of compound chlorhexidine on bac-terial biofilm.SPSS13.0 was used for statistical analysis.Results SEM observation in the experimental group added compound chlorhexidine showed the failure to form a good biofilm.The crystal violet assay showed that compound chlorhexidine had the inhibitory effect on plaque biofilms （P 〈0.05）.CLSM showed that bio-film thickness was decreased after adding compound chlorhexidine （P 〈0.05）.Conclusion As one of the common oral gargle, compound chlorhexidine has a certain inhibition on the formation of Porphyromonas gingivalis bacteria biofilm.
作者
王一宇
孙盼盼
史艳芬
宋静
WANG Yiyu SUN Panpan SHI Yanfen SONG Jing(School of Stomatology,Jining Medical University Jining Medical University,Jining 272067 ,China)
出处
《济宁医学院学报》
2016年第6期395-398,共4页
Journal of Jining Medical University
关键词
复方氯己定
牙龈卟啉单胞菌
生物膜
Compound chlorhexidine
Porphyromonas gingivalis
Biofilm
