摘要
目的:探讨血浆HMGB1、IFN-γ、IL-4和CD4^+T细胞表面TLR4的表达对免疫性血小板减少症(ITP)的影响。方法:选取我院2014年10月至2015年10月确诊为ITP的患者25例,同时选取健康人20例为对照组;采用酶联免疫吸附法(ELISA)检测两组血浆HMGB1、IFN-γ和IL-4的水平;流式细胞术检测CD4^+T细胞表面TLR4的表达水平;分析不同参数之间的关系。结果:ITP组治疗前血浆HMGB1水平显著高于对照组(t=4.259,P<0.01),治疗后降低至接近对照组(t=1.267,P>0.05);ITP组治疗前、后血浆IFN-γ检测值与对照组差异均无统计学意义(P>0.05,P>0.05);ITP组治疗前血浆IL-4水平非常显著低于对照组(t=5.708,P<0.01),治疗后明显高于对照组(t=2.107,P=0.01);ITP组治疗前血浆IFN-γ/IL-4比值非常显著高于对照组(t=5.436,P<0.01),治疗后显著降低且略低于对照组;ITP组治疗前后TLR4表达均非常显著低于正常对照组(P<0.01);ITP患者HMGB1水平与CD3^+的含量呈正比(r=0.824,P<0.01),与CD4^+的含量无明显关系(r=0.074,P>0.05),与CD8^+的含量呈正比(r=0.844,P<0.01),与IL-4的含量呈正比(r=0.784,P<0.01),与IFN-γ的含量呈反比(r=-0.814,P<0.01),与IFN-γ/IL-4比值呈反比(r=-0.887,P<0.01),与TLR4表达水平呈正比(r=0.772,P<0.01)。结论:ITP患者HMGB1和TLR4的表达水平较高,临床治疗可以通过靶向控制其表达,以缓解病情并达到治疗目的。
Objective：To investigate the effects of plasma HMGB1,IFN-γ,IL-4 and CD4^＋ T cell surface TLR4 expression on the immune thrombocytopenia（ITP）.Methods：Twenty- five patients diagnosed as ITP in our hospital from October 2014 to October 2015,and 20 healthy persons as controls were selected.The ELISA was used to detect the plasma levels of HMGB1,IFN-γ and IL-4 in 2 groups;the flow cytometry was used to detect the expression level of TLR4 on the surface of CD4^＋ cells.The relatienship between different parameters was analyzed.Results：Before treatment,the plasma HMGB1 level in ITP group was significantly higher than that in control group（t =4.259,P〈 0.01）,while after treatment it significantly decreased and close to level in control group（t = 1.267,P〉0.05）.The plasma IFN-γ level detected in ITP group before and after treatment was not significantly different from level in control group（P〈0.05）,while the IL-4 level in ITP group before treatment was significantly lower than that in control grup（t= 2.107,P〈0.05）,but the IL-4 level in ITP group after treatment was significantly higher than that in control group（t= 2.107,P〈0.05）.The plasma IFN-γ/IL-4 ratio in ITP group before treatment was significantly higher than that in control group（t =5.436,P 〈0.01）,but it obviously decreased and was slightly lower than that in control group after treatment.The expression level of TLR4 in ITP group before and after treatment was all significantly lower than that in control group（P 〈0.01）.The HMGB1 level in ITP group was directly proportional to the CD3 -＋ content（r =0.824,P 〈0.01）,however it not significantly related with CD4^＋ content（r = 0.074,P〉0.05）,but than the HM GB1 level in ITP group was directly proportional to CD8^＋ content（r = 0.844,P〈0.01） and to IL-4 content（r =0.784,P〈0.01）,and was inversely proportional to IFN-γ level（r =- 0.814 P 0.01）,and also was inversely proportional to IFN-γ/IL-4 ratio（r =-0.887 P〈0.01）,and directly
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2016年第6期1837-1841,共5页
Journal of Experimental Hematology
基金
国家临床重点专科建设项目(卫办医政函[2011]873号)
青海省人才小高地项目(青人才字[2014]12号)
