摘要
以16年生马占相思优树为材料建立高效的组培技术体系,最佳的消毒方法是0.1%升汞处理9 min,75%酒精处理15 s,此时存活率和出芽率最高,分别为66.67%和78.00%。最佳外植体为第3-5腋芽茎段,每年高温少雨的8月为最佳的采条季节,优树枝条建立采穗圃中采集的外植体萌芽率高于来自母株枝条外植体的萌芽率,最佳芽诱导培养基是改良MS+6-BA 0.5 mg/L+蔗糖30 g/L。于8月取最佳外植体,最佳消毒方式处理后,接种于最佳芽诱导培养基,此时芽诱导率最高,为93.33%。最佳增殖培养基为:MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+Ac 0.05 g/L+蔗糖20 g/L,增殖倍数为3.96,7次继代培养,增殖倍数稳定,平均增殖倍数为3.08。最佳生根培养基为1/2 MS+IBA 0.5 mg/L+NAA 0.5 mg/L+蔗糖30 g/L,最高生根率为94.36%。建立的优树组培体系对今后加快马占相思良种选育及优质苗木大量扩繁具有重要作用。
The explants from 16-year-old elite tree of Acacia mangium were used to establish the efficient tissue culture technique. The maximum survival rate(66.67%) and germination rate(78.00%) appeared when treatment with 0.1% mercuric chloride 9 min, 75% ethanol 15 s, which was optimal. 3-5 node of shoot as explants were optimal. It was the optimal season to gather explants in August when it was high temperature and rainless.Explants germinated from shoots of cutting orchard had a higher germination rate relative to those from shoots of seed trees. The optimal bud inducing medium is modified MS medium with 6-BA 0.5 mg/L and sucrose 30 g/L,with the highest germination rate of 93.33% when the optimal explants(gathered in August, 3-5 node of shoot from cutting orchard) were cultured in it. The optimal proliferate medium was MS medium with 6-BA 0.5 mg/L, NAA0.1 mg/L, Ac 0.05 mg/L1 and sucrose 20 g/L, proliferation rate reached maximum(3.96), after subculture 7 times with stable proliferation rate, the average proliferation time was 3.08. The optimal rooting medium was 1/2 MS medium with IBA 0.5 mg/L, NAA 0.5 mg/L, sucrose 30 g/L,with the highest rooting rate(94.36%). The technique established in this paper would supply technology basic and guarantee for Acacia mangium rapid propagation to meet the seedling needs for plantations in China.
出处
《分子植物育种》
CAS
CSCD
北大核心
2016年第4期986-996,共11页
Molecular Plant Breeding
基金
国家"十二五"科技支撑计划项目(2012BAD01B0402)资助
关键词
马占相思
成年优树
组培快繁
Acacia mangium
Mature elite tree
Tissue culture
