摘要
目的观察六味地黄丸含药血清对人血管内皮细胞Eahy926增殖及其ERK/Nrf2-HO-1信号通路的影响。方法 CCK-8检测大鼠5%、10%、20%空白血清与5%、10%、20%含药血清对Eahy926细胞增殖的影响,并用Western-blotting方法检测10%含药血清对Eahy926细胞ERK/Nrf2-HO-1信号通路分子蛋白表达的影响。结果六味地黄丸含药血清可以促进Eahy926细胞的增殖;在Eahy926细胞中,10%六味地黄丸含药血清能够促进ERK的磷酸化、Nrf2核转位和HO-1蛋白的表达,明显高于无大鼠血清组和10%空白血清组。结论 10%六味地黄丸含药血清诱导ERK磷酸化介导Nrf2入核和HO-1蛋白的表达。
Objective To investigate the effect of drug-containing serum of Liuwei Dihuang pills on the ERK / Nrf2-HO-1 pathway in Eahy926 cells. Methods The proliferation of Eahy926 cells was detected by CCK-8 method. Western blotting was used to determine the protein expression of the ERK / Nrf2-HO-1pathway. Results The drug-containing serum of Liuwei Dihuang pills promoted the proliferation of Eahy926 cells. The levels of ERK phosphorylation,Nrf2 nuclear translocation and HO-1 expression in Eahy926 cells treated with 10% drug-containing serum of Liuwei Dihuang pills were significantly higher than that of the control and blank groups. Conclusion 10% drug-containing serum of Liuwei Dihuang pills promotes Nrf2 nuclear translocation and expression of HO-1 via inducing ERK phosphorylation.
作者
吕小辉
孙佳瑜
蔡智刚
张瑞鹏
单爱云
LYU Xiaohui SUN Jiayu CAI Zhigang ZHANG Ruipeng SHAN Aiyun(Shenzhen Futian District Traditional Chinese Medicine Hospital, Guangdong 518033, China Key Lab for New Drugs Research of TCM, Shenzhen Research Institute of Tsinghua University, Shenzhen 518067, China)
出处
《广东药学院学报》
CAS
2016年第5期618-621,共4页
Academic Journal of Guangdong College of Pharmacy
基金
深圳市科技研发基金(JCYJ20140414145007216)
