摘要
目的了解江苏省淮安市男男性行为(men who have sex with men,MSM)人群感染HIV-1的基因亚型分布和分子特征。方法以现住址为淮安的HIV-1确证感染者为研究对象,利用巢式逆转录聚合酶链反应(reverse transcription polymerase chain reaction,RT-PCR)方法扩增env基因片段,测序并进行分子进化分析。结果淮安市MSM人群感染的HIV-1基因亚型有5种,分别是CRF01-AE(68.21%,118/173)、CRF07-BC(16.76%,29/173)、CRF08-BC(0.58%,1/173)、CRF55-01B(1.16%,2/173)及B亚型(13.29%,23/173)。系统进化分析显示淮安流行的毒株与云南、辽宁、广西、江苏及湖南分离的同源性高,在进化树上与对应亚型的参考株聚集成簇。流行的优势亚型CRF01-AE中共检测出58个单倍型,单多样性指数(0.999)和核苷酸的多样性指数(0.150)显示淮安市株具有较高的遗传多样性。结论淮安市MSM人群感染的HIV-1存在多样性,其中CRF55-01B为首次在淮安市MSM感染人群发现,CRF01-AE是淮安市MSM人群中感染的主要流行株。
Objective To gain more insights into genotype and evolutionary characteristics of HIV-1 infection among men who have sex with men （MSM） in Huai＇ an, Jiangsu. Methods Local confirmed cases in Huai＇ an were collected. HIV-1 env genes were amplified by nested reverse transcription polymerase chain reaction （RT-PCR）from （RNA） DNA, followed by sequencing and molecular evolutionary analysis. Results The distribution of HIV-1 infection genotypes among MSM were as follows ： CRF01 -AE （ 68. 21% , 118/173 ） , CRF07-BC （ 16. 76% , 29/173 ） , CRF08-BC （0. 58% , 1/ 173） ,CRF55-01 B（ 1.16%, 2/173） and B （13. 29%, 23/173）. Comparison of env gene sequences indicated that the HIV-1 strains circulating in Huai＇ an had high nucleotide identity with strains in Yunnan, Liaoning, Guangxi, Jiangsu and Hunan and clustering with their subtypes together on the phylogenetic tree. Overall, 58 haplotypes of CRFO1-AE genotype were defined among individuals and CRF01-AE genotype of Huai＇ an isolates were equipped with a high haplotypic diversity index （0. 999） and low nueleotide diversity index （0. 150） also indicated the high genetic diversities. Conclusions HIV- 1 genotypes are diverse in Huai＇ an. CRF55-01B was firstly detected among MSM in Huai＇ an. CRF01-AE is the dominant epidemic sub-type in MSM.
出处
《中华疾病控制杂志》
CAS
CSCD
北大核心
2016年第12期1231-1235,共5页
Chinese Journal of Disease Control & Prevention
基金
淮安市科技计划项目(HAS2015019-5)
淮安市预防医学科研基金(hayf201504
hayf201505)
