摘要
CO_2地质封存过程复杂,存在CO_2泄漏风险,进而会影响土壤生态系统,为研究地质封存CO_2泄漏应对措施,以玉米为研究对象,采用人工控制释放CO_2系统研究了玉米对地质封存CO_2泄漏的响应特征,并应用高通量测序技术分析了玉米土壤细菌群落结构及遗传多样性。结果表明:土壤CO_2浓度为30×104μL·L^(-1)(30 cm埋深处CO_2浓度)时,玉米表现出较好的适应能力,当CO_2泄漏量增加到40×104μL·L^(-1)以上时,玉米叶片等各项生长指标均表现为受胁迫特征;高通量测序结果显示试验共获得4个土壤样本的70 948个OTUs,但仅3.32%为4种泄漏条件下土壤所共有;遗传信息表明土壤细菌多样性和丰富度变化与CO_2泄漏浓度密切相关;变形菌门、拟杆菌门和酸杆菌门等为土壤优势菌群,而酸杆菌门在土壤CO_2浓度达40×104μL·L^(-1)下相对丰度大幅增加,暗示其可用作地质封存CO_2泄漏对土壤生态系统影响的指示菌。可见泄漏的CO_2会影响农作物生长及土壤细菌群落特征,而变化的土壤细菌多样性会影响农田生态系统功能等。
The CO_2 geological storage technology carries a risk of the sealed CO_2 leakage due to the complexity of the storage process, which might affect soil ecosystem. The response of maize to the leakage scenarios of sealed CO_2 was studied by an artificial CO_2 release system,and the bacterial community and genetic diversity in soils were analyzed by high-throughput sequencing technology. The results showed that：Maize adapted to 30×104μL·L^（-1）CO_2scenarios; but more than 40×104μL·L^（-1）CO_2 showed a stress on some physiological indexes of maize leaves and so on. There were a total of 70948 OTUs for four soil samples by high-throughput sequencing technology, but only 3.32% of OTUs were common under different CO_2 leakage scenarios. Changes in soil bacterial diversity and richness were related to CO_2 leakage levels.Proteobacteria, Bacteroidetes, and Acidobacteria etc. were dominant species in soils. A significant increase in relative abundance of Aci-dobacteria under 40×104μL·L^（-1）CO_2 concentration suggested that it might be an indicator bacteria for the impact of sealed CO_2 leakage on soil ecosystem. Overall, CO_2 leakage would affect crop growth and soil microbial community, and changes in microbial diversity might affect functions of agro-ecosystem. This study is important for the establishment of geological CO_2 sequestration leakage coping technology.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2016年第12期2440-2448,共9页
Journal of Agro-Environment Science
基金
国家自然科学基金项目(41302207)
陕西省自然科学基金项目(2014JM5212)
准噶尔等盆地二氧化碳地质储存综合地质调查(DD20160307)
关键词
CO2地质封存
CO2泄漏
玉米
生理特征
高通量测序
土壤细菌群落
geological storage of CO2
CO2 leakage
maize
physiological characteristics
high-throughput sequencing
soil bacterial community
