摘要
为了探讨白鱼(Anabarilius)腐败菌产生物胺的能力,釆用聚合酶链式反应技术检测腐败菌的组氨酸、赖氨酸、鸟氨酸和酪氨酸的脱羧酶基因,应用反相高效液相色谱技术测定接种腐败菌培养基和无菌白鱼鱼块中生物胺含量,并对主要腐败菌的生物胺产量因子进行分析。结果表明:8种腐败菌均含有至少1种氨基酸脱羧酶基因;接种不同腐败菌的液体培养基和无菌白鱼鱼块均检测到多种生物胺,其中Morganella sp.wf-1和Morganella sp.wf-2具有很强的产腐胺、尸胺和组胺能力,使得接种这两种腐败菌的无菌白鱼鱼块中腐胺、尸胺和组胺含量、单胺、二胺、生物胺指数和总生物胺含量等指标在腐败过程中均显著高于对照组和接种其他腐败菌的无菌白鱼鱼块。同时,Morganella sp.wf-1和Morganella sp.wf-2的腐胺、尸胺、组胺、单胺、二胺、生物胺指数和总生物胺的产量因子均高于其他腐败菌。结果表明,Morganella sp.wf-1和Morganella sp.wf-2是白鱼腐败过程中产生物胺的主要腐败菌。
In order to analyze the biogenic amines-producing capacity of different spoliage microorganisms isolated from artificially inoculated Whitefish, the polymerase chain reaction（PCR） was applied to detect the genes encoding histidine, lysine, ornithine and tyrosine decarboxylase. Meanwhile, high performance liquid chromatography（HPLC） was applied to detect the biogenic amines produced by different spoliage microorganisms grown in liquid medium and whitefish tissue. The yield factors of biogenic amines of the major spoliage organisms were also analyzed. The results showed that each of 8 spoliage microorganisms contained at least one amino acid decarboxylase gene. Multiple biogenic amines were detected in the liquid medium and whitefish tissue by HPLC. Among the tested strains, Morganella sp.wf-1 and Morganella sp.wf-2 showed higher productivity of putrescine, cadaverine and histamine, which resulted in higher contents of putrescine, cadaverine, histamine, momoamine, diamine, biogenic amine index and total biogenic amine in Whitefish tissue inoculated with Morganella sp.wf-1 and Morganella sp.wf-2 compared with whitefish tissue inoculated with other spoilage organisms and control group during the spoilage process. Meanwhile, Morganella sp.wf-1 and Morganella sp.wf-2 showed higher yield factors of putrescine, cadaverine, histamine, momoamine, diamine, biogenic amine index and total biogenic amine compared with other spoilage organisms. These results indicated that Morganella sp.wf-1 and Morganella sp.wf-2 were the major spoilage microorganisms that can produce biogenic amines during the spoilage of Whitefish.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第23期196-204,共9页
Food Science
基金
江苏省水产三新工程项目(Y2015-29)
关键词
白鱼
腐败菌
生物胺
产量因子
Whitefish
spoliage organism
biogenic amine
yield factors
