摘要
目的研究脂氧素对糖尿病大鼠心肌缺血再灌注损伤心肌凋亡细胞及表达的影响。方法按照体重将SD大鼠随机分为3组:假手术组、模型组和实验组。先建立糖尿病大鼠模型,然后实验组给予脂氧素0.1 mg·kg^(-1),再建立心肌缺血再灌注损伤模型。计算细胞凋亡指数;用免疫印迹法和RT-PCR法检测心肌组织Bax蛋白、半胱氨酸天冬氨酸蛋白酶3(Caspase-3);用酶联免疫吸附法检测肿瘤坏死因子-α(TNF-α)、白细胞介素^(-1)(IL^(-1))、白细胞介素-6(IL-6)、白细胞介素-8(L-8)含量。结果假手术组、模型组与实验组的心肌细胞凋亡指数分别为(4.96±0.61)%,(16.72±1.96)%,(8.95±0.99)%,模型组的心肌细胞凋亡指数高于假手术组,实验组的心肌细胞凋亡指数低于模型组,差异有统计学意义(均P<0.05)。模型组大鼠的血清TNF-α为(52.29±6.84)ng·L^(-1)、IL^(-1)为(24.49±3.74)ng·L^(-1)、IL-6为(314.59±45.64)ng·m L^(-1)、IL-8为(223.49±34.59)ng·m L^(-1);均高于假手术组的TNF-α为(19.62±2.42)ng·L^(-1)、IL^(-1)为(9.18±1.05)ng·L^(-1)、IL-6为(114.52±14.54)ng·m L^(-1)、IL-8为(84.59±10.12)ng·m L^(-1);而实验组血清TNF-α为(30.39±4.65)ng·L^(-1)、IL^(-1)为(13.52±1.95)ng·L^(-1)、IL-6为(189.46±23.49)ng·m L^(-1)、IL-8为(135.19±17.85)ng·m L^(-1),模型组与假手术比较或实验组与模型组比较,差异均有统计学意义(均P<0.05)。模型组、假手术和实验组大鼠的心肌组织中mRNA Bax表达量分别为249.55±31.23,100.00±14.52,155.49±18.49,而Caspase-3表达量分别为264.95±34.52,100±16.09,170.19±19.52;Bax蛋白表达量分别为221.38±19.45,100.00±15.68,135.54±16.79;Caspase-3蛋白表达量分别为305.69±35.62,100.00±16.10,145.62±21.34。模型组高于假手术组、而实验组低于模型组,组间比较差异均有统计学意义(均P<0.05)。结论脂氧素对糖尿病大鼠心肌缺血再灌注损伤具有保护作用,能够减少细胞凋亡数目并抑制凋亡基因与蛋白表达
Objective To study the effect of lipoxin on apoptosis and expression of myocardial cells in diabetic rats with myocardial ischemia - reperfusion injury. Methods SD rats were randomly divided into three groups： Sham operation group, model group and experimental group. Diabetic model and myocardial ischemia reperfusion injury model were made. The diabetic rats in experimental group were given lipoxin 0.1 mg·kg^-1 aftermodeling. The apoptosis index was calculated. Bax protein of myocardial tissure, cysteinyl aspartate specific protease （Caspase -3 ） were detected by Western blotting and RT- PCR. The levels of tumor necrosis factor-α （TNF-α）, interleukin 1 （IL- 1 ）, IL- 6, IL- 8 were detected using ELISA. Results The apoptosis indexes in sham operation group, model group, experimental group were （4.96 ± 0. 61 ） %, （ 16. 72 ± 1.96 ） %, （ 8.95 ± 0. 99 ） %, respectively. The apoptosis index of model group was higher than that of sham operation group, while experimental group was lower than model group（all P〈0.05）. The myocardial TNF-α was（19.62 ±2.42）ng·L^-1,IL-1 was （9.18±1.05）ng·L^-1, IL-6 was（114.52 ±14.54）ng·mL^-1,IL-8 was（84.59 ±10. 12）ng·mL^-1 in sham operation group. Compared with sham operation group, the myocardial TNF -α （52.29 ± 6.84） ng·L^-1, IL-1（24.49±3.74）ng·L^-1 IL-6（314.59±45.64）ng·mL^-1, IL-8（223.49±34.59）ng·mL^-1 in model group were higher（ P 〈 0.05 ）. Compared with model group, the myocardial TNF - α（ 30.39 ± 4.65 ） ng·L^-1,IL-1 （13.52±1.95）ng·L^-1, IL-6（189.46±23.49） ng·mL^-1,IL-8（135. 19±17.85）ng·mL^-1 in experimental group were lower （P 〈 0.05 ）. The expression levels of mRNA Bax in model group, sham operation group and experimental group were 249.55±31.23,100.00±14.52,155.49±18.49, respectively; while expression levels of Caspase - 3 were 264.95 ±34.52,100.00 ± 16.09,170. 19± 19.52, respectively. The expression levels of protein Bax in model group, sham operation grou
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2016年第24期2273-2276,共4页
The Chinese Journal of Clinical Pharmacology
基金
广西自然科学基金资助项目(2011GXNSFA018215)
关键词
脂氧素
缺血再灌注损伤
凋亡
炎症
lipoxin
ischemia reperfusion injury
apoptosis
inflammation
