摘要
建立纤维素酶固态发酵与生物预处理相耦合工艺。实验优化固态发酵条件,测定发酵基质结晶度及酶解糖化得率。结果表明3 g稻草粉为基质,0.5%淀粉为碳源,1%蛋白胨为氮源,0.5%芦丁,初始pH值为5,发酵14d,褐腐真菌Piptoporus betulinus产CMC酶活力达到76.46 U/g,滤纸酶活力达到7.75 U/g;酶解糖化阶段减少外源纤维素酶量36.05%;P.betulinus降解固态基质中的无定型纤维素,暴露结晶纤维素,提高发酵基质的酶解糖化得率184%。
A novel solid state fermentation （SSF） process with Piptoporus betulinus for cellulase production coupled with biological pretreatment was established. In this study, the conditions of SSF were optimized, and then the crystallinity index and the reducing sugar yield of fermented substrate was measured. The results showed that under the prepared optimum conditions of 0.5% starch, 1% peptone, 0.5% rutin and pH 5, using 3 g rice straw as substrate, the CMC enzyme activity and filter paper enzyme activity from SSF by Piptoporus betulinus could reach 76.46 U/g and 7.75 U/g, respectively. The crystalline cellulose of the fermented substrate was exposed due to the degradation of amorphous cellulose by P. betulinus. Moreover, the reducing sugar yield was increased by 184%, yet the cellulase dosage was decreased by 36.05% during the process of enzymatic hydrolysis of the fermented substrate.
出处
《纤维素科学与技术》
CAS
CSCD
2016年第4期19-24,75,共7页
Journal of Cellulose Science and Technology
基金
国家自然科学基金项目(31560524)
内蒙古自治区自然科学基金项目(2015BS0201)
内蒙古大学2014年博士引进科研启动经费(21400-5145138)
关键词
褐腐真菌
桦剥管孔菌
固态发酵
纤维素酶
酶解糖化得率
brown rotten fungi
Piptoporus betulinus
solid state fermentation
cellulase
reducing sugar yield
