摘要
目的:探索同型半胱氨酸(Hcy)诱导的内皮祖细胞(EPCs)的功能障碍能否被黄酒所逆转。方法:密度梯度离心法从大鼠骨髓细胞悬液中获取单个核细胞,将其接种在人纤维连接蛋白包被的培养板,诱导单个核细胞向EPCs分化,孵箱中培养7 d后,收集贴壁细胞用于实验。激光共聚焦显微镜鉴定Di I-ac-LDL和FITC-UEA-I双染色阳性细胞被认定是正在分化的EPCs。上述细胞培养24 h后收集样品,采用MTT比色法、Transwell小室、凋亡试剂盒和体外血管生成试剂盒分别观察EPCs的活力、迁移能力、凋亡和体外血管生成能力。结果:与对照组相比,Hcy干预下EPCs的活力、迁移和体外血管生成能力均显著减弱(P<0.01);黄酒或红酒的干预则能明显改善Hcy对EPCs上述功能的影响(P<0.01);进一步与对照组比较后,发现黄酒或红酒的干预还能使EPCs的活力、迁移和体外血管生成能力较对照组也有明显改善(P<0.05);乙醇组EPCs的上述功能较Hcy组没有明显的变化。各组对EPCs的凋亡没有影响。结论:Hcy能显著减弱EPCs的活力、迁移能力和体外血管生成能力,小剂量的黄酒能改善EPCs的上述功能。
AIM：To investigate whether Chinese yellow wine has influences on homocysteine（ Hcy）-induced dysfunction in rat endothelial progenitor cells（ EPCs）.METHODS：Rat bone marrow was extracted to harvest mononuclear cells（ MNCs） by density gradient centrifugation.The MNCs were plated on fibronectin-coated culture dishes,and were induced into EPCs by EGM-2 complete medium supplemented with cell growth factor.The adherent cells were collected 7 d later for all studies.EPCs were characterized as adherent cells double positive for Di I-ac-LDL uptaking and lectin binding by direct fluorescent staining under a laser scanning confocal microscope.The viability,migration,apoptosis and in vitro vasculogenic activity of the EPCs were determined by MTT assay,Transwell chamber assay,apoptosis kit and in vitro vasculogenesis kit,respectively.RESULTS：Compared with control group,the viability,migration and in vitro vasculogenic capacity of the EPCs in Hcy group were significantly decreased（ P〈0.01）.Compared with Hcy group,yellow wine group and red wine group both significantly improved the viability,migration and in vitro vasculogenic capacity of Hcy-induced EPCs（ P〈0.01）.Compared with control group,yellow wine group and red wine group both significantly improved the above-mentioned functions of EPCs（ P〈0.05）.However,no significant difference of apoptosis in all groups was observed.CONCLUSION：Hcy may result in dysfuction of EPCs.Treatment with yellow wine improves Hcy-induced EPC functions.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2016年第12期2216-2221,共6页
Chinese Journal of Pathophysiology
基金
卫生部科学研究基金-浙江省医药卫生重大科技计划项目(No.Wkj2011-2-018)
浙江省自然科学基金资助项目(No.LY14H020002)
关键词
黄酒
动脉粥样硬化
内皮祖细胞
同型半胱氨酸
Yellow wine
Atherosclerosis
Endothelial progenitor cells
Homocysteine
