摘要
目的:探讨荔枝核总黄酮(TFL)对急性肺损伤大鼠胆碱能抗炎通路的影响。方法:将60只SD大鼠随机分成6组:空白对照组、模型对照组、地塞米松3 mg·kg^(-1)组、荔枝核总黄酮100,50,25 mg·kg^(-1)组,每组10只大鼠,连续灌胃给药7 d。末次给药30 min后,腹腔注射20%酵母混悬液(5 mL·kg^(-1))建立大鼠急性肺损伤模型。末次注射2 h后,处死大鼠,摘眼球取血法取血,分离出血清,待测。测定大鼠左肺组织进行的湿干比重(W/D)。测定肺组织中乙酰胆碱转移酶(ChAT)、乙酰胆碱酯酶(AChE)的活性。ELISA法检测血清中白介素^(-1)β(IL-1β)、一氧化氮(NO)、乙酰胆碱(ACh)、肿瘤坏死因子-α(TNF-α)的含量。蛋白免疫印迹(Western blot)法测定大鼠肺组织中核因子-κB(NF-κB)的蛋白含量。结果:与空白对照组相比,模型组大鼠湿干比重上升,IL-1β、NO、TNF-α及NF-κB的含量提高,ACh的含量下降,ChAT和AChE的活性减弱(P<0.05)。与模型组相比,荔枝核总黄酮组的大鼠肺组织湿干比重下降,IL-1β、NO、TNF-α及NF-κB的含量下降,ACh的含量升高,ChAT和AChE的活性增加(P<0.05)。结论:荔枝核总黄酮可通过调节胆碱能抗炎通路,抑制炎症反应的发展,使受损肺组织得到保护。
OBJECTIVE To investigate protective effects of total flavonoids of litchi（TFL）on cholinergic anti-inflammatory pathway in rats with acute lung injury.METHODS Sixty SD rats were randomly divided into normal group,model group,dexamethasone group（3 mg·kg（-1））,100,50,25 mg·kg（-1） of TFL groups,each with 10 rats respectively.Rats were intragastrically gavaged for 7 days.Normal group and model group were given saline.Thirty minutes after last gavage,rats with acute lung injury were created by intraperitoneally injecting 20% yeast suspension every 12 h for two times.Wet and dry proportion,ChAT and AChE in lung tissues were determined.Serum TNF-α,IL（-1）β,ACh and NO contents were detected by ELISA.Protein expression of NF-κB was measured by Western blot.RESULTS Compared with model group,wet and dry proportion of lung tissues,TNF-α,IL（-1）β,NO and NF-κB contents were decreased by TFL.ACh,ChAT and AChE content increased（P〈0.05）.CONCLUSION TFL can inhibit development of inflammatory response by regulating cholinergic anti-inflammatory pathway,and protect the damaged lung tissues.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2016年第23期2051-2054,共4页
Chinese Journal of Hospital Pharmacy
基金
国家自然科学青年基金(编号:81500633)
关键词
荔枝核总黄酮
急性肺损伤
胆碱能抗炎通路
大鼠
total flavonoids of litchi
acute lung injury
cholinergic anti-inflammatory pathway
rats
